Molecular cloning

Zebrafish spata2 is expressed at early developmental stages

Developmental Biology / Computational Biology / In Situ Hybridization / Biological Sciences / Spermatogenesis / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence

Zebrafish spata2 is expressed at early developmental stages

Developmental Biology / Computational Biology / In Situ Hybridization / Biological Sciences / Spermatogenesis / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence

4-Methylphthalate catabolism in Burkholderia (Pseudomonas) cepacia Pc701: a gene encoding a phthalate-specific permease forms part of a novel gene cluster

Microbiology / Multidisciplinary / Nucleic acid hybridization / Plasmids / Membrane transport proteins / Molecular cloning / Benzoic Acid / Base Sequence / Burkholderia Cepacia / Reading Frames / Molecular cloning / Benzoic Acid / Base Sequence / Burkholderia Cepacia / Reading Frames

A doxycycline-inducible, tissue-specific aromatase-expressing transgenic mouse

Technology / Prostate Cancer / Transgenic Mice / Biological Sciences / Adipose tissue / Humans / Mice / Female / Animals / Male / Drinking Water / Plasmids / Molecular cloning / Mammary gland / Doxycycline / Transgenic / In Vitro Studies / Salivary Gland / Transgenic Mouse Technology / Transgene Expression / Full Length Movies / Salivary Glands / Tissue Specificity / Aromatase Activity / Humans / Mice / Female / Animals / Male / Drinking Water / Plasmids / Molecular cloning / Mammary gland / Doxycycline / Transgenic / In Vitro Studies / Salivary Gland / Transgenic Mouse Technology / Transgene Expression / Full Length Movies / Salivary Glands / Tissue Specificity / Aromatase Activity

Zebrafish wnt3 is expressed in developing neural tissue

Signal Transduction / Biological Sciences / Phylogeny / Animals / Neurons / Molecular cloning / Body Patterning / Zebrafish / Nervous System / Amino Acid Sequence / Molecular cloning / Body Patterning / Zebrafish / Nervous System / Amino Acid Sequence

Sema7A is a Potent Monocyte Stimulator

Immunology / Flow Cytometry / Cytokines / Humans / Animals / Glycoproteins / Scandinavian / Neutrophils / Molecular cloning / Monocytes / Lipoproteins / Superoxides / Glycoproteins / Scandinavian / Neutrophils / Molecular cloning / Monocytes / Lipoproteins / Superoxides

Human testis expresses a specific poly(A)-binding protein

RNA / Polymers / Western blotting / Biological Sciences / Cell line / Environmental Sciences / Humans / Male / Nucleic Acids / tESTIS / Molecular cloning / RNA-binding proteins / Amino Acid Sequence / Base Sequence / Blood Proteins / Environmental Sciences / Humans / Male / Nucleic Acids / tESTIS / Molecular cloning / RNA-binding proteins / Amino Acid Sequence / Base Sequence / Blood Proteins

P fimbriae of Escherichia coli : Molecular cloning of DNA fragments containing the structural genes

Biological Sciences / Escherichia coli / Molecular cloning / Sperm Dna Fragmentation

Xenopus Phospho-CDK7/Cyclin H Expressed in Baculoviral-Infected Insect Cells

Mass Spectrometry / Cell Cycle / Cell line / Animals / Threonine / Phosphorylation / Molecular cloning / Protein expression and purification / Xenopus laevis / Protein Expression / Protein Kinase / Biochemistry and cell biology / Phosphorylation / Molecular cloning / Protein expression and purification / Xenopus laevis / Protein Expression / Protein Kinase / Biochemistry and cell biology

Lys80p ofSaccharomyces cerevisiae, previously proposed as a specific repressor ofLYS genes, is a pleiotropic regulatory factor identical to Mks1p

Microbiology / Industrial Biotechnology / Transcription Factors / Saccharomyces cerevisiae / Yeast / Molecular cloning / Lysine / Molecular cloning / Lysine

Unusual pseudosubstrate specificity of a novel 3,5-dimethoxyphenol O-methyltransferase cloned from Ruta graveolens L

Kinetics / Escherichia coli / Temperature / Enzyme / Metals / Molecular cloning / Substrate Specificity / Amino Acid Profile / Molecular weight / Amino Acid Sequence / S-Adenosylmethionine / Hydrogen-Ion Concentration / Phloroglucinol / Ruta / Biochemistry and cell biology / Molecular cloning / Substrate Specificity / Amino Acid Profile / Molecular weight / Amino Acid Sequence / S-Adenosylmethionine / Hydrogen-Ion Concentration / Phloroglucinol / Ruta / Biochemistry and cell biology

Melanoma vaccine candidates from chimeric hepatitis B core virus-like particles carrying a tumor-associated MAGE-3 epitope

Biotechnology / Industrial Biotechnology / Environmental Biotechnology / Cancer Vaccines / Hepatitis B / Humans / Medical Biotechnology / Melanoma / CpG islands / Molecular cloning / Amino Acid Sequence / Base Sequence / Humans / Medical Biotechnology / Melanoma / CpG islands / Molecular cloning / Amino Acid Sequence / Base Sequence

LXR-α genomics programmes neuronal death observed in Alzheimer’s disease

Degenerative Diseases / Apoptosis / Epigenomics / Humans / Reactive Oxygen Species / Withanolides / Heme Oxygenase / Medical Physiology / Neurons / Neuroblastoma / Molecular cloning / Cellular Automaton Model / Neuronal Death / Human Serum / Orphan Nuclear Receptors / Recombinant Proteins / Alzheimer Disease / Biochemistry and cell biology / Gene Expression Regulation / Withanolides / Heme Oxygenase / Medical Physiology / Neurons / Neuroblastoma / Molecular cloning / Cellular Automaton Model / Neuronal Death / Human Serum / Orphan Nuclear Receptors / Recombinant Proteins / Alzheimer Disease / Biochemistry and cell biology / Gene Expression Regulation

Intra- and Extracellular  -Galactosidases from Bifidobacterium bifidum and B. infantis: Molecular Cloning, Heterologous Expression, and Comparative Characterization

Multidisciplinary / Escherichia coli / Applied / Applied Environmental Microbiology / Enzyme / Molecular cloning / Amino Acid Profile / Molecular Mass / Amino Acid Sequence / Heterologous Expression / Molecular cloning / Amino Acid Profile / Molecular Mass / Amino Acid Sequence / Heterologous Expression

Gliap--a novel untypical L-asparaginase localized to rat brain astrocytes

Neurochemistry / Brain / Animals / Astrocyte / Rat Brain / Astrocytes / Molecular cloning / L-asparaginase / Rats / Glutathione Transferase / Amino Acid Sequence / Base Sequence / Neurosciences / Astrocytes / Molecular cloning / L-asparaginase / Rats / Glutathione Transferase / Amino Acid Sequence / Base Sequence / Neurosciences

A novel chimeric MOMP antigen expressed in Escherichia coli, Arabidopsis thaliana, and Daucus carota as a potential Chlamydia trachomatis vaccine candidate

Chlamydia trachomatis / Escherichia coli / Electroporation / Animals / Genetic transformation / Arabidopsis / Solubility / Molecular cloning / Protein expression and purification / Rabbits / Amino Acid Sequence / Agrobacterium / Recombinant Proteins / Daucus Carota / Biochemistry and cell biology / Immunoblotting / Arabidopsis / Solubility / Molecular cloning / Protein expression and purification / Rabbits / Amino Acid Sequence / Agrobacterium / Recombinant Proteins / Daucus Carota / Biochemistry and cell biology / Immunoblotting
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