Site-directed Mutagenesis

Processing DNA molecules as text

Polymerase Chain Reaction / Text Processing / Process Planning / Error Correction / Computer Control / Homologous Recombination / Large Scale / Systems and Synthetic Biology / Site-directed Mutagenesis / Dna Synthesis / DNA fragmentation / Homologous Recombination / Large Scale / Systems and Synthetic Biology / Site-directed Mutagenesis / Dna Synthesis / DNA fragmentation

comH, a Novel Gene Essential for Natural Transformation of Helicobacter pylori

Genetics / Bacteriology / Helicobacter pylori / Horizontal Gene Transfer / Biological Sciences / Gram-negative bacteria / Genome sequence / Peptic Ulcer / Natural Transformation / Site-directed Mutagenesis / H Pylori / Gene Transfer / DNA fragmentation / Gram-negative bacteria / Genome sequence / Peptic Ulcer / Natural Transformation / Site-directed Mutagenesis / H Pylori / Gene Transfer / DNA fragmentation

Aerial development in Streptomyces coelicolor requires sortase activity

Molecular Microbiology / Biological Sciences / Molecular / Streptomyces coelicolor / Substrate Specificity / Cysteine endopeptidases / Site-directed Mutagenesis / Cysteine endopeptidases / Site-directed Mutagenesis

Structural Basis for NADH/NAD+ Redox Sensing by a Rex Family Repressor

Gene regulation / Gene expression / Macromolecular X-Ray Crystallography / Molecular Mechanics / Cell and Molecular Biology / Biological Sciences / Surface plasmon resonance / Gram Positive Bacteria / DNA binding / Protein Conformation / NAD / Site-directed Mutagenesis / Oxidation-Reduction / Biological Sciences / Surface plasmon resonance / Gram Positive Bacteria / DNA binding / Protein Conformation / NAD / Site-directed Mutagenesis / Oxidation-Reduction

Protein PknE, a novel transmembrane eukaryotic-like serine/threonine kinase from Mycobacterium tuberculosis

Signal Transduction / Mycobacterium tuberculosis / Protein Kinases / Mutation / Threonine / Phosphorylation / Biochemical / Plasmids / Enzyme / Lysine / Glutathione Transferase / Protein Phosphorylation / Protein Kinase / Cytoplasm / Site-directed Mutagenesis / Full Length Movies / Biochemistry and cell biology / Cell Membrane / Phosphorylation / Biochemical / Plasmids / Enzyme / Lysine / Glutathione Transferase / Protein Phosphorylation / Protein Kinase / Cytoplasm / Site-directed Mutagenesis / Full Length Movies / Biochemistry and cell biology / Cell Membrane

Hydrolysis of organophosphorus insecticides by in vitro modified carboxylesterase E3 from Lucilia cuprina

Zoology / Kinetics / Drosophila melanogaster / Insecticides / Insecticide Resistance / Cell line / Glutamate / Humans / Mutagenesis / Resistance / Animals / Acetylcholinesterase / Enzyme / Anions / Isoenzymes / Diptera / Amino Acid Profile / Insect Biochemistry and Molecular Biology / Malathion / Amino Acid Sequence / Amino Acid Substitution Rates / Hydrolysis / Kinetic Parameter / Site-directed Mutagenesis / Insect Biochemistry / Biochemistry and cell biology / Cell line / Glutamate / Humans / Mutagenesis / Resistance / Animals / Acetylcholinesterase / Enzyme / Anions / Isoenzymes / Diptera / Amino Acid Profile / Insect Biochemistry and Molecular Biology / Malathion / Amino Acid Sequence / Amino Acid Substitution Rates / Hydrolysis / Kinetic Parameter / Site-directed Mutagenesis / Insect Biochemistry / Biochemistry and cell biology

Processing DNA molecules as text

Polymerase Chain Reaction / Text Processing / Process Planning / Error Correction / Computer Control / Homologous Recombination / Large Scale / Systems and Synthetic Biology / Site-directed Mutagenesis / Dna Synthesis / DNA fragmentation / Homologous Recombination / Large Scale / Systems and Synthetic Biology / Site-directed Mutagenesis / Dna Synthesis / DNA fragmentation

Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design

Kinetics / Biotechnology / Industrial Biotechnology / Gene expression / Protein Engineering / Environmental Biotechnology / Biocatalysis / Biological Sciences / Protein Design / Mutation / Escherichia coli / Medical Biotechnology / Temperature / Hydrogen Bonding / Theoretical Models / Active site / Protein Secondary Structure Prediction / Buffers / Substrate Specificity / Bioreactors / Phosphates / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Protein Binding / Galactose Binding / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Oxidation-Reduction / Molecular Sequence Data / Environmental Biotechnology / Biocatalysis / Biological Sciences / Protein Design / Mutation / Escherichia coli / Medical Biotechnology / Temperature / Hydrogen Bonding / Theoretical Models / Active site / Protein Secondary Structure Prediction / Buffers / Substrate Specificity / Bioreactors / Phosphates / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Protein Binding / Galactose Binding / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Oxidation-Reduction / Molecular Sequence Data

Binding of a Cyano- and Fluoro-containing Drug Bicalutamide to Cytochrome P450 46A1: UNUSUAL FEATURES AND SPECTRAL RESPONSE

Water / Kinetics / Biological Chemistry / Biological Sciences / Brain / Humans / Mutation / Nitriles / Cholesterol / Animals / Iron / Spectrophotometry / Heme / CHEMICAL SCIENCES / Cattle / Molecular Conformation / Protein Binding / Antineoplastic Agents / Site-directed Mutagenesis / Humans / Mutation / Nitriles / Cholesterol / Animals / Iron / Spectrophotometry / Heme / CHEMICAL SCIENCES / Cattle / Molecular Conformation / Protein Binding / Antineoplastic Agents / Site-directed Mutagenesis

The amino acid transport system y+L/4F2hc is a heteromultimeric complex

Physiology / RNA / Humans / Low Dose / Animals / Non Functional Requirement / Medical Physiology / Amino Acids / Xenopus laevis / Cysteine / Protein Conformation / Membrane Protein / Oocytes / Site-directed Mutagenesis / Biochemistry and cell biology / Gene Expression Regulation / Mercuric Chloride / Non Functional Requirement / Medical Physiology / Amino Acids / Xenopus laevis / Cysteine / Protein Conformation / Membrane Protein / Oocytes / Site-directed Mutagenesis / Biochemistry and cell biology / Gene Expression Regulation / Mercuric Chloride

Transport of Preproteins by the Accessory Sec System Requires a Specific Domain Adjacent to the Signal Peptide

Bacteriology / Protein Folding / Biological Sciences / Membrane transport proteins / Protein Secondary Structure Prediction / SIGNAL PEPTIDE / Amino Acid Sequence / Amino Acid Substitution Rates / Site-directed Mutagenesis / Molecular Sequence Data / SIGNAL PEPTIDE / Amino Acid Sequence / Amino Acid Substitution Rates / Site-directed Mutagenesis / Molecular Sequence Data

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