Carbohydrate-Test - Amino Acid-Test

Carbohydrate-Test  The Molisch-Test:Procedure: 1- Add 1 ml Sample. 2- Add 2 Drop Molisch reagent. 3- Add 1 ml H2SO4. (Monosaccharide’s give a rapid positive test)

 Barfoed-Test:Procedure: 1- Add 0.5 ml of The Sample. 2- Add 1 ml of Barfoed reagent. 3- Boil 3-5 min in Water Bath. (monosaccharide’s give positive result using Barfoed's reagent.)

 Seliwanoff’s-Test:Procedure: 1- Add 2 Drop Sample to 2.5 ml Seliwanoff’s Reagent. 2- Warm 1-2 min in Water Bath. 3-The red Color within 2 min indicates positive Result Ketoses (Fructose , Sucrose). two common sugars that give a positive test Ketoses (Fructose , Sucrose)  Bials-Test:Procedure:1- Add 0.5 ml Sample to 1.5 ml of Bials Reagent. 2- Heat Boiling in Water Bath. 3- The Formation of a Blue-Green Color positive Result for pentose. compounds (pentosans) will give a positive test.

 Benedict’s – Test:Procedure:1- Add 2 ml Sample in Test Tube. 2- Add 1 ml Benedict’s Reagent. 3- Boil 10-15 min in Water Bath.

 Starch – Test:Procedure:1- Add 2 ml Sample in Test Tube. 2- Add 2 ml Iodin.

(1 ml) (1 ml)

Amino Acid-Test  Ninhydrin- Test:- Is a test for Amino Acids with a free -NH2 group. Procedure:1- Add 1 ml Sample A,B,C in Test Tube. 2- Add 5 Drop Reagent Ninhydrin. 3- Heating 2 min in Water Bath.

 Sakaguchi-Test:- (Contain Arginine) Procedure:1- Add 1 ml sample in Test Tube. 2- Add 2 ml %10 NaOH. 3- Add 3 Drop α- naphthol 4- yields a reddish wine color when arginine is present.

 Xanthoproteic test ( mulder test):- Activated benzene ring Procedure:1- Add 0.5 ml Sample in tube. 3- Heat 3 min in Water Bath.

2- Add 0.5 ml HNO3 4- Yellow