Expression of cytotoxic mediators (perforin, granzyme B, FAS, and FAS-l) in renal allograft biopsies Expressão de mediadores citotóxicos (perforina, granzima B, FAS e FAS-l) em biópsias de aloenxerto renal

Expression of cytotoxic mediators (perforin, granzyme B, FAS, and FAS-l) in renal allograft biopsies

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ORIGINAL ARTICLE

Expression of cytotoxic mediators (perforin, granzyme B, FAS, and FAS-l) in renal allograft biopsies Expressão de mediadores citotóxicos (perforina, granzima B, FAS e FAS-l) em biópsias de aloenxerto renal Therezinha Gauri Leitão 1, Luís Eduardo Becker2, Ivone Braga de Oliveira3, Flávia Ramos de Siqueira4, Maria Regina Teixeira Araújo5, João Egídio Romão Junior 6, Hugo Abensur7, Irene de Lourdes Noronha8

ABSTRACT Objectives: To analyze the in situ expression of perforin, granzyme B, FAS -L and FAS in renal allograft biopsies by means of immunohistochemistry and correlate these findings with the degree of histologic rejection and allograft outcome. Methods: Ninety-six allograft biopsies were divided into three groups: acute rejection (n = 56), chronic rejection (n = 31), and cases with stable renal function (no rejection; n = 9). The expression of perforin, granzyme B, FAS-L, and FAS was evaluated by immunohistochemistry. Results: A significantly higher expression of perforin and granzyme B was observed in acute rejection biopsies (4.83 ± 0.65 and 30.05 ± 7.93 cells/mm2) compared to chronic rejection biopsies (0.71 ± 0.13 and 11.4 ± 3.84 cells/mm2; p < 0.001, and p