HIF-1alpha overexpression improves mesenchymal stem cells angiogenesis potential

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Abstracts

Poster – [A-10-1148-1] Association of − 762C/T P2X7 receptor gene polymorphism with susceptibility to tuberculosis in Zahedan, southeast Iran Gholamreza Bahari, Mohsen Taheri, Abdolkarim Moazeni-Roodi, Mohammad Naderi, Mohammad Hashemi Zahedan, Iran E-mail addresses: [email protected] (G. Bahari), [email protected] (M. Taheri), [email protected] (A. Moazeni-Roodi), [email protected] (M. Naderi), [email protected] (M. Hashemi) Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is a public health problem and remains a major cause of morbidity and mortality around the world. One third of the earth's population is infected with TB but only 10% individuals infected will develop the disease.This fact indicate that genetic factors play an important role in susceptibility to TB. The purinergic P2X7 receptors shown to induce killing of intracellular M. tuberculosis, followed by apoptosis of the infected macrophage. Methods: This case–control study was done on 151 PTB patients (58 males, 93 females; age; 48.77 ± 21.33) and 151 healthy subjects (50 males, 101 females; age; 45.23 ± 16.04). Genotype analysis of −762C/T P2X7 receptor gene polymorphism was done using amplification refractory mutation system-PCR (ARMS-PCR). The frequency of −762 CC, CT, and TT were 47.7% (72/151), 35.8%(54/151) and 16.5%(25/151) in patients, While the frequency in healthy subjects were 68.2%(103/151), 27.8%(42/151), 4%(6/151) respectively. Results: We found that CT genotype is a risk factor for TB (OR= 1.893, 95%CI = 1.112–3.043, p =0.021) as well as TT genotype (OR= 5.961, 95%CI = 2.327–15.27, p < 0.0001). Frequency of T allele in PTB was 34.4%(104/302), and in healthy subjects was 17.9%(54/302). T allele is a risk factor for TB (OR = 2.412, 95%CI = 1.653–3.521, p