http://audioslides.elsevier.com/getvideo.aspx?doi=10.1016/j.yexcr.2014.12.003

August 4, 2017 | Autor: Mohammed Mansour | Categoria: Cancer, Cancer Biology, Cancer Research, P53 , Stem Cells and Colon Cancer
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Oncomine database: SATB2 expression Normal vs. Cancer

Legend 0. No value (7) 1. Normal Colon (5) 2. Cecum Adenocarcinoma (17) 3. Colon Adenocarcinoma (41) 4. Colon Mucinous Adenocarcinoma (13) 5. Rectal Adenocarcinoma (8) 6. Rectal Mucinous Adenocarcinoma (4) 7. Rectosigmoid Adenocarcinoma (10)

1 Murphy & Courtneidge (2011) Nature Reviews Molecular Cell Biology

Figure 1 C

FLAG

FLAGSATB2

400

FLAG

350

Phalloidin

FLAG-SATB2 Phalloidin

300 250

200

*

150 100

Cortactin

Cortactin

50

0

Merge

D Cortactin

FITC-gelatin

Merge

70

% of cells with invadopodia

Number of invaded cells/field

B

60 50 40 30 20 10 0

*

Figure 2

L. Jin, 2011

A

HCT116

B

150

110

GFP 50 30

110

SATB2

Relative mRNA level of Palladin

kDa

1.2 1 0.8 0.6 0.4 0.2

C

0

HCT116

CW-2

COLO 320

kDa

β-actin

200 150 116

97

Palladin 66

β-actin

Figure 3 HCT116

A

B shRNA:

kDa

CRC cells:

200 150 116 97

Palladin

200 150 97

Palladin

66

66

α-tubulin

β-actin

C shCtrl

shPALLD #1 shPALLD #2

100 90 80

Migrating distance (arbitrary unit)

0 hr

70

shCtrl shPALLD #1 shPALLD #2

60

12 hr

50 40

**

*

24

36

30

24 hr

20 10

36 hr

0 0

12

Time (h)

*

Figure 4 A

shCtrl

shPALLD #1

Cortactin

Phalloidin

Merge

70

% of cells with invadopodia

B

60 50 40

*

30

*

20 10 0 shCtrl

shPALLD #1

shPALLD #2

shPALLD #2

Figure 6

A

kDa

B

FLAG.SATB2

FLAG.SATB2/ GFP-palladin

FLAG.SATB2/ GFP-palladin (wt)

D

200 150

GFP

Cortactin

Merge

PALLD 97 400

97

GFP

30

Number of invaded cells/field

116

*

350 300 250 200 150 100 50

Actin

0 FLAG.SATB2/ GFP-palladin

% of cells with invadopodia

FLAG.SATB2

ECM degradation

SATB2

Palladin Cytoskeletal dynamics

Migration signaling

70

*

60 50 40 30 20 10 0 FLAG.SATB2/ GFP

FLAG.SATB2/ GFP-palladin

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