New insight into propylparaben in vitro toxicity: DNA oxidative damage

July 9, 2017 | Autor: V. Labrador | Categoria: Oxidative Damage, ENVIRONMENTAL SCIENCE AND MANAGEMENT
Share Embed


Descrição do Produto

Abstracts / Toxicology Letters 196S (2010) S37–S351

samples. For this purpose, 98 healthy volunteers were studied by standard PCR-RFLP technique. Cd and Pb were analyzed by Graphite Furnace Atomic Spectrometry; Fe and Zn were analyzed by Flame Atomic Absorption Spectrometry. As a result; no significant association was found between the IVS4+44C/A SNP in the DMT1 and Pb (p = 0.309), Zn (p = 0.698), and Fe (p = 0.391) levels in the placenta samples but statistically significant association was detected with the Cd concentration (p = 0.001). The average Cd concentration of the placenta samples was 17.20 ± 16.31 ppb. The determined average Cd levels for the individuals with CC genotypes were 14.11 ± 12.17 ppb, whereas the average values were 15.42 ± 13.60 ppb and 29.97 ± 24.80 ppb for the individuals with CA and AA genotypes, respectively. doi:10.1016/j.toxlet.2010.03.551

P202-016 Assessment of the in vivo genotoxicity of a new formulation of the Biolarvicide Bactivec A. Curbelo 1 , A. Mancebo 1 , T. Molier 2 , M.E. Arteaga 1 , C. González 1 , R. Grandía 1 , A.M. Bada 1 , Y. Rivero 1 , A. García 1 , M. Legró 1 , F. Contreras 1 1

CENPALAB, Cuba, 2 LABIOFAM, Cuba

The use of biological control agents has become a useful alternative for the reduction of the use of chemical insecticides. Biolarvicide Bactivec is produced in Cuba and possesses the active biological agent, Bacillus thuringiensis var israelensis. In order to evaluate the genotoxicity of Bactivec, an in vivo battery test was used: micronucleus, chromosome aberrations and sperm morphology assays. A dose of 6.45 × 108 spores was administered per animal via oral administration. Bone marrow cells were collected 24 h after a 2-day treatment for the micronucleus assay, and 24 h after a unique treatment, for the chromosome aberration assay, using Cyclophophamide as positive control. Sperm cells were collected at 5 weeks from the first of five administrations for the sperm morphology test, using Acrylamide as positive control. Bactivec failed to show either a significative increase of micronucleated polychromatic erythrocytes, chromosomal aberrations, or sperm abnormalities. Acute oral administration of a high dose of Bactivec did not produce mutagenic effects in bone marrow or sperm cells. doi:10.1016/j.toxlet.2010.03.552

P202-017 New insight into propylparaben in vitro toxicity: DNA oxidative damage J.M. Pérez Martín, A. Peropadre, Ó. Herrero, P. Fernández Freire, V. Labrador, M.J. Hazen Universidad Autónoma de Madrid, Spain Propyl p-hydroxybenzoate, commonly referred to as propylparaben, is the most frequently used preservative to inhibit microbial growth and extend shelf life of a range of consumer products. The objective of this study was to provide further insight into the toxicological profile of this compound, because of the current discrepancy in the literature with regard to parabens safety. The Vero cell line, derived from the kidney of green monkey, was selected to evaluate the adverse effects of propylparaben using a set of mechanistically relevant endpoints for detecting cytotoxicity and genotoxic activity. Our results demonstrate that exposure

S161

to the compound for 24 h causes changes on cell proliferation rates rather than on cell viability. A significant and dose-dependent decline in the percentage of mitotic cells was observed from the lowest concentration tested, mainly due to cell cycle arrest at the G0/G1 phase. Immunodetection techniques revealed that induction of DNA double-strand breaks and oxidative damage underlie the cytostatic effect observed in Vero-treated cells. Additional studies are in progress to extend these findings that define a novel mode of action of propylparaben in cultured mammalian cells. doi:10.1016/j.toxlet.2010.03.553

P202-018 Nascent polypeptide-associated complex in ribosomes plays a key role in protection against adriamycin toxicity in budding yeast T. Takahashi, A. Naganuma. Graduate School of Pharmaceutical Sciences, Tohoku University, Japan Adriamycin (ADM) is an anthracycline antitumor antibiotic used in cancer chemotherapy. Recently, the involvement of ribosomal proteins in the development of ADM resistance in cancer cells has been reported, suggesting that ribosomes play an important role in the manifestation of ADM toxicity. However, the extent and nature of ribosomal involvement in this process remain largely unknown. To elucidate the role of ribosomes in the manifestation of ADM toxicity, we performed a screen for ribosome-binding proteins involved in ADM sensitivity using budding yeast as a eukaryotic model. This revealed that ADM toxicity was enhanced by loss of the Egd1 or Egd2 subunits of the nascent polypeptide-associated complex (NAC). NAC is a heterodimer consisting of alpha (Egd2) and beta (Egd1 or Btt1) subunits, and is known to be involved in the translocation of nascent polypeptides into mitochondria or endoplasmic reticulum and in transcriptional activation in the nucleus. Because the loss of the Btt1 subunit had no effect on ADM sensitivity, the NAC conformation responsible for resistance to ADM appears to be the Egd1/Egd2 complex. We also found that the mutant yeast cells lacking N-terminal region of Egd1, which is required for interaction between NAC and ribosome, are hypersensitive to ADM. Moreover, no correlation between decreased transcriptional activity and ADM sensitivity was observed in the mutant yeast cells. Our results suggest that dysfunction of NAC in ribosome, but not in nucleus, might enhance ADM toxicity. doi:10.1016/j.toxlet.2010.03.554

P202-019 Effect of IL-6 −174 G/C polymorphism on toxic metal and trace element levels in placenta Z. Kayaaltı, D. Tekin, S. Yalc¸ın, V. Aliyev, E. Söylemez, G. Kurtay, T. Söylemezo˘glu Ankara University, Turkey The placenta is a privileged organ of fetal origin to provide nutrients to the fetus from the mother. During pregnancy the need for essential micronutrients such as iron and zinc increases due to the requirement of a growing fetus. Fe deficiency is common and caused by a serious consequences in fetal growth retardation. Similarly, Cu deficiency, although not so common, also has destructive effects. On the other hand, the altering Fe status affects on

Lihat lebih banyak...

Comentários

Copyright © 2017 DADOSPDF Inc.