P15 Clinical and dermoscopical diagnosis of micro-melanoma

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Melanoma Research 2010, Vol 20 e-Supplement A

cancer was completed. Data on anatomical location, histologic subtype of the tumor and Breslow thickness was obtained for each patient from pathology reports. Results Twelve out of 434 consecutive patients with a primary cutaneous melanoma (2.8%) were subsequently diagnosed with a second primary melanoma. Two of these cases also had a family history of melanoma. Eleven patients had a total of 2 primary melanomas while one patient had three primary tumors. The mean Breslow thickness of the index tumor was 1.46 mm (range 0.52– 3.4 mm) and of the 2nd primary tumor was 0.59 mm (0.16–0.95 mm). The mean age at diagnosis was 38 years (range 18–57 years). Seven patients (58%) had Fitzpatrick’s skin phototype II. History of high cumulative sun exposure reflected by occupational sun exposure and the presence of actinic keratoses was reported in 3 (25%) and 2 (16%) patients respectively, while recreational exposure and increased sun exposure during childhood (> 5 weeks/ year) was reported in 8 patients (67%). Eleven patients had dysplastic nevi and 8 out of 11 (86%) had a prominent mole pattern ( > 50 common nevi). Conclusion The incidence of multiple primary melanomas in Greek patients appears to be lower than that reported in several studies of Northern European populations. Although our sample is small, our findings suggest that patients with multiple nevi or dysplastic nevi and a history of high intermittent exposure to UVR are at a higher risk of developing a secondary primary melanoma. Familial history was not a predominant feature in our MPM patients.

P15 Clinical and dermoscopical diagnosis of micro-melanoma E. Tolomio, F. Crippa, D. Moglia, A. Bono and M. Santinami Istituto Nazionale Tumori, Milan, Italy

Introduction The aim of this study was to describe the clinical and dermoscopic diagnosis in a series of cutaneous melanomas with a diameter r 3 mm (micro-melanomas). Methods From July 1997 to December 2006 we observed 54 micro-melanomas. Each lesions was subjected to both clinical and dermoscopical evaluation before surgery. The clinical and dermoscopic feature as well as the corresponding diagnosis were recorded. Dermoscopic criteria for diagnosis of malignancy were those of Menzies. Results The typical lesion presents as a small, dark, often black macule, generally evenly coloured with well defined borders. Dermoscopic features more represented were irregular pseudopods, atypical network and radial streaming. Sensitivity of clinical and dermoscopical diagnosis was respectively 41 and 74%.

Conclusion Detenction of very small melanoma is feasible by accurate visual inspection. Dermoscopy appears to be a an efficient aid to the diagnosis of micro-melanomas, provided that clinicians are aware of this type of lesions and maintain the index of suspicion at a high level.

P16 In-transit metastase or primary melanoma? N. Demirkan, I. Gokalan Kara, S. Ergin and H. Tosun Pamukkale University, Denizli, Turkey

We report here a case of cutaneous malignant melanoma of ear which has a polemical macroscopic appearance. A 36 year old man presented with polypoid pigmented and ulcerated lesion measuring 1–2 cm in diameter and second macular lesion at 0.3 cm distance of primary lesion. The second lesion measures 0.8–0.3 cm in diameter. There were two metastatic lymph nodes in the resection material. Histopathological examination of each lesion ensured to distinguish the primary tumor which was macular lesion and the other polypoid lesion as in-transit metastase. Finally we concluded the stage of case as pT1N3Mx. We discussed the importance of histopathological staging of malignant melanoma before the decision of treatment.

P17 Comparative genomic hybridization (CGH) analysis of a distinct variant of melanocytic nevi, named deep penetrating blue nevi L. Held, J. Bauer and C. Garbe Centre for Dermatooncology, Department of Dermatology, University Hospital Tuebingen, Germany

Background Deep penetrating nevus (DPN) is a distinct variant of melanocytic nevi. DPN possesses a distinct architecture and cytology with deep dermal and subcutaneous involvement. DPNs are melanoma simulators. Comparative genomic hybridization (CGH) is a method to assess the presence of DNA copy number changes in tumors. Copy number aberrations are frequently found in solid cancers. Thus, CGH has been suggested as a diagnostic aid in melanoma. Objective sTo evaluate the presence of copy number aberrations in DPNs and melanomas mimicking DPN. Methods and results DNAs from six cases of DPN were micro-dissected and purified from paraffin embedded tissue. Tumors were then analyzed by comparative genomic hybridization (CGH) using Agilent 105 k CGH arrays. In three out of six cases we found no chromosomal aberrations. However, the other three tumors showed complex