British Journal of Cancer (2012) 107, 1883–1891 & 2012 Cancer Research UK All rights reserved 0007 – 0920/12
www.bjcancer.com
Serum apolipoprotein C-II is prognostic for survival after pancreatic resection for adenocarcinoma A Xue1, JW Chang1, L Chung2, J Samra1, T Hugh1, A Gill3, G Butturini4, RC Baxter2 and RC Smith*,1 1
Department of Gastrointestinal Surgery, University of Sydney, Kolling Institute of Medical Research, St Leonards NSW 2065, Australia; 2Hormones and Cancer Group, Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards NSW 2065, Australia; 3Department of Anatomical Pathology, Royal North Shore Hospital, St Leonards NSW 2065, Australia; 4Surgical Department, University of Verona, Piazzale le. Scuro, Verona 10 37134, Italy
BACKGROUND:
Pancreaticoduodenectomy remains a major undertaking. A preoperative blood test, which could confidently predict the benefits of surgery would improve the selection of pancreatic cancer patients for surgery. This study aimed to identify protein biomarkers prognostic for long-term survival and to validate them with clinico-pathological information. METHODS: Serum from 40 preoperative patients was used to train for predictive biomarkers using surface-enhanced laser desorption/ ionisation time-of-flight mass spectrometry (SELDI), and the results were verified on 21 independent samples. Two predictive proteins were identified by tryptic peptide mass fingerprinting and sequencing, and validated on serum from another 57 patients by enzymelinked immunosorbent assay (ELISA). The influence of these proteins on growth and invasion of two cancer cell lines was tested in-vitro. RESULTS: The SELDI panel of m/z 3700, 8222 and 11 522 peaks predicted o12 months’ survival (ROC AUC: 0.79, 0.64–0.90; Po0.039). When CA19-9 was added, the ROC AUC increased to 0.95 (0.84–0.99; Po0.0001). The six subjects in the verification group who died within 12 months were correctly classified. The m/z 8222 and 11 522 proteins were identified as Serum ApoC-II and SAA-1, respectively. In the validation samples, ELISA results confirmed that ApoC-II was predictive of survival (Kaplan–Meier Po0.009), but not SAA-I. ApoC-II, CA19-9 and major-vessel involvement independently predicted survival. ApoC-II and SAA-1 increased cell growth and invasion of both cancer cell lines. CONCLUSION: Serum ApoC-II, CA19-9 and major-vessel invasion independently predict survival and improves selection of patients for pancreaticoduodenectomy. British Journal of Cancer (2012) 107, 1883–1891. doi:10.1038/bjc.2012.458 www.bjcancer.com & 2012 Cancer Research UK Keywords: pancreatic neoplasms; pancreaticoduodenectomy; prognosis; apolipoproteins; survival; biomarkers
Surgical resection and adjuvant chemotherapy for pancreatic cancer provide the best chance of long-term survival (Castellanos et al, 2011). The outcome appears to be independent of pylorus preservation (Akizuki et al, 2008), but may be improved by more extensive resection of mesopancreatic tissue (Samra et al, 2006) and vascular resection (Tang et al, 2011). Despite the introduction of these improvements over the last decade, one-fifth of patients do not survive 12 months; an unsatisfactory result, given that the survival time includes prolonged recovery from major surgery and that palliative treatments may have achieved the same outcome (Distler et al, 2010). When faced with the decision to undergo surgery, it is important for patients to be given an accurate prognosis, particularly regarding the chance of long-term survival. Prognosis generally relies on established pathological classification such as the TNM staging of malignant tumours (TNM) (American Joint Committee on Cancer, 2009). To further improve the prognosis, the expression of a range of tissue proteins has been reported to have prognostic value in cohorts of patients undergoing pancreatic resection, but none has been studied in a prospective randomised manner (Jamieson et al, 2011), and their usefulness in the clinic remains unproven. Plasma biomarkers are *Correspondence: Professor R Smith; E-mail:
[email protected] Received 19 June 2012; revised 10 September 2012; accepted 12 September 2012
more available in the preoperative setting, with Carbohydrate Antigen 19-9 (CA 19-9) being the most commonly used for diagnosis and for prognosis in clinical practice. However, although reviews have supported the prognostic use of CA 19-9 (Mehta et al, 2010), others have reported it to be unreliable (Kondo et al, 2010). An elevated preoperative value of CA 19-9 was found not to be prognostic of survival, while its continual elevation in the postoperative setting was a significant indicator of poor outcome (Hata et al, 2012). Carbohydrate Antigen 19-9 is frequently elevated in benign conditions and more so in the jaundiced patient (Molina et al, 2012). Other serum markers of the host’s response to cancer such as human C-reactive protein (CRP) (Pine et al, 2009), serum amyloid A-1 (SAA-1) (Firpo et al, 2009) and the neutrophil to lymphocyte ratio (Bhatti et al, 2010) are also reported to predict survival. Such studies justify an exploratory examination of serum in an attempt to improve prediction of outcome, particularly in the era of extensive surgery and neoadjuvant therapy. One potential source of such biomarkers is the pool of low-abundance small proteins in serum. Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (SELDI) was used to discover diagnostic protein biomarkers, including ApoA-II and ApoC-I (Xue et al, 2010), which were not previously recognised. Further, the SELDI technique has also revealed biomarkers of pancreatic cancer-related outcomes such as invasiveness (Valkovskaya et al,
Molecular Diagnostics
ApoC-II predicts outcome of resection for PCa A Xue et al
1884 2007), responsiveness to gemcitabine (Cao et al, 2010) and cachexia (Felix et al, 2011). It is therefore possible that SELDI could identify prognostic markers of long-term survival in the serum. This study uses the SELDI platform to discover markers of prognosis in a training set of serum samples, and verifies the markers by SELDI in an independent set of serum samples. The prognostic capability of these proteins is then assessed using ELISA, along with other predictors of survival, in a further independent validation set of serum samples.
MATERIALS AND METHODS Patients and sera The study was approved by the Northern Sydney Health Human Research Ethics Committee, Sydney, Australia (HREC 909 228M) and the University of Verona, Ethics Committee, Verona, Italy. The training set Sera from 40 consecutive patients about to undergo pancreaticoduodenectomy for pancreatic ductal carcinoma (PDCa) at the Uniersity of Verona (UniVer) from 2006–2007 were prospectively collected and stored at 80 1C as the ‘training set’ until analysed by SELDI. The verification set Sera from 21 consecutive patients (the ‘verification set’), collected before pancreatic resection at Royal North Shore Hospital (RNSH) in 2007 were analysed by SELDI.
Molecular Diagnostics
The validation set Further 82 serum samples from subjects at RNSH from 2009 to 2010, consisting of sera from 57 PDCa patients before pancreatic resection, 13 with unresectable (advanced) pancreatic cancer (APDCa) and 12 healthy volunteers (HV), which were controls for the analysis technique were collected prospectively as a ‘validation set’. Clinical and pathological data for the validation set were prospectively recorded in a Microsoft Access database. The analysis of the data was delayed until 12 months after surgery. Two ‘validation set’ patients were lost to follow-up at 4 and 10 months, and their data were censored at the last review day, when they were alive. Tumours were synoptically classified according to defined pathological prognostic criteria by one of the authors, AG, as previously described (Gill et al, 2009). Carbohydrate Antigen 19-9 and CRP were measured by enzyme-linked immunosorbent assay (ELISA) and the neutrophil–lymphocyte ratio was recorded for the validation-set patients.
Preparation of serum and proteinchip arrays for SELDITOF MS Serum was separated into aliquots 30 min after collection and stored at 80 1C. From each sample, 20 ml was diluted 1 : 1 with denaturing buffer (8 M urea per 1% CHAPS), then centrifuged at 12 000 rpm for 5 min. Protein levels were measured by Micro BCH protein assay kits (Thermo Scientific, Chicago, IL, USA), and the amount of buffer added to the samples adjusted to provide 2 mg per spot. The supernatant was diluted to 1 : 25 with 10% acetonitrile (ACN)/0.1% trifluoroacetic acid (TFA). Treated samples were applied to hydrophobic (H50) proteinchip arrays and processed, as previously described (Xue et al, 2010). The proteinchip arrays were analysed using the Bio-Rad Enterprise SELDI System (Bio-Rad, Hercules, CA, USA).
SELDI-TOF MS Analysis Duplicate spectra were obtained in the mass/charge (m/z) range 2500–75 000 with a laser intensity set at 220 (arbitrary units), detector sensitivity at 8 and a signal to noise ratio of 5%. The m/z value for each peak was determined using external calibration with British Journal of Cancer (2012) 107(11), 1883 – 1891
standards (Sigma–Aldrich, St Louis, MO, USA) which are the following: bovine insulin (5734.51 þ 1H), equine cytochrome c (12 361.96 þ 1H), equine apomyoglobin (16 952.27 þ 1H) and rabbit muscle aldolase (39 212.28 þ 1H). Spectra were analysed using ProteinChip Software, Version 3.1 (Bio-Rad).
Protein peak identification Serum samples were separated into six fractions eluted at pH 9, 7, 5, 4, 3 and the organic phase using the ProteinChip Serum Fractionation Kit (Bio-Rad). The fractions of interest were immediately applied to a C18 reverse-phase HPLC column eluted with a gradient of 15–60% ACN in 0.1% TFA. Eluted fractions containing the protein peaks of interest were lyophilised and then subjected to 1D SDS–PAGE, staining with Coomassie Blue to visualise the proteins. The bands containing the putative proteins of interest were cut and sent to the Australian Proteome Analysis Facility (APAF, Macquarie University, Sydney, Australia) who used nano-LC-ESI MS/MS (nano-liquid chromatography electro-spray ionisation tandem MS) for protein identification by MS sequencing. Peak lists were generated using MASCOT script in Analyst 2.0 (AB Sciex, Framingham, MA, USA). The MS/MS spectra with 410 peaks were identified using a centroid identification protocol, deisotoped, and the peak areas 41% maximum reported. The search parameters used by MASCOT were: database: SwissProt 55.2 (362782 sequences; 130497792 residues); taxonomy: homo sapiens (human) (19117 sequences); type of search: MS/MS ion search; enzyme: trypsin; mass values: monoisotopic; protein mass: unrestricted; peptide mass tolerance: ±300 ppm; fragment mass tolerance: ±0.6 Da; maximum missed cleavages: 1; criteria for acceptance: MudPIT scoring, significant threshold Po0.05, peptide ion score 425 and bold red only. Protein identities were confirmed by a SELDI immunoadsorption approach. An ApoC-II antibody (AF-4497, R&D System, McKinley, Minneapolis, MN, USA) and a SAA-1 antibody (sc-59679, Santa Cruz Biotechnology, Santa Cruz, CA, USA), both diluted 1 : 2000, were separately conjugated to RS100 proteinchips and immunoreactive proteins were allowed to bind, then the chips were washed and analysed by SELDI.
Measurement of serum levels of CA19-9, SAA-1 and ApoC-II by ELISA The levels of CA 19-9, ApoC-II and SAA-1 in serum were measured using a CA 19-9 ELISA Kit (Alpha Diagnostic International, San Antonio, TX, USA), AssayMax ApoC-II ELISA kit (AssayPro, St Charles, MO, USA) and Human SAA Assay Kit (Invitrogen, Carlsbad, CA, USA), respectively. Sample dilution was fivefold for CA 19-9 and 200-fold for ApoC-II and SAA-1. The absorbance values for the three analytes were measured at 450 nm using a Microplate Reader (Tecan, Salzburg, Austria) within 10 min. Duplicate samples were measured blind in a random order with controls and the average coefficient of variation was 3.7%.
Pancreatic cancer cell lines and cell proliferation assay Two human pancreatic cancer cell lines, CFPAC-1 (ATCC CRL1918; ATCC, Manassas, VA, USA) and PANC-1 (ATCC CRL-1469) were kindly provided by Professor Barry Allen (St George Hospital, Sydney, Australia). CFPAC-1 cells were cultured in RPMI 1640 containing 10% foetal bovine serum (FBS; Gibco BRL, Gaithersburg, MD, USA). PANC-1 cells were cultured in Dubecco’s Modified Eagle’s Medium (DMEM) with 10% FBS. Cells were grown in a humidified (37 1C, 5% CO2) incubator and passaged upon reaching 80% confluence at a ratio of 1 : 3. The effect of the candidate prognostic markers on cell growth was determined in CFPAC-1 and PANC-1 cells by the colorimetric 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, & 2012 Cancer Research UK
ApoC-II predicts outcome of resection for PCa A Xue et al
1885 Table 1
Demographic and pathological details of the study groups Patient groups
Sex (M:F) Age (years) Bilirubin (mmol l 1) ALP (IU l 1) AST (IU l 1) ALT (IU L 1) Albumin (g l 1) CA 19-9 (U ml 1) Number of Whipples procedures Total pancreatectomy Portal vein resection Added arterial resection Adjuvant chemotherapy R0 R1 R2 Stage I–II:III Grade I–II:III T 1, 2, 3, 4 N 1, 2, 3 Median survival (m)
Training set
Verification set
Validation set
23 : 17
12 : 9
33 : 24
Mean
Range
Mean
Range
Mean
Range
60.5 66 194 82 110
57.7–70.2 16–243 93–361.5 30–135 25–248
128 32 8
28–703
70.9 60 193 66 87 36.5 130 21
28–80 9–189 69–433 43–133 35–218 33–41 147–229
67 24** 170 63 76 31 96 57
61–75 9–41 66–524 12–400 8–622 23–38 31–266
3 29 24 11 5 25 : 15 25 : 15 4, 21, 11, 4 24, 12, 4 15
11–22
4 7 10 4 13 : 8 16 : 5 5, 10, 6, 1 10, 11 17.3
11–23
22 4 42 25 31 1 41 : 16 39 : 18 5, 6, 40, 6 19, 10, 28 14
10–18
Sigma–Aldrich) assay method described previously (Xue et al, 2009).
100
80
Statistics and bioinformatics For SELDI analysis, spectra were normalised to the total ion current between 2500 and 75 000 m/z and peaks were detected using the biomarker wizard utility (Bio-Rad). In the training set, univariate analysis by Mann–Whitney test at Po0.05 was used to distinguish patients who survived more or o12 m. Six-fold crossover validation and logistic regression were used to predict 412-m survival after surgery. The discriminatory power for each marker was characterised by receiver operating characteristics (ROC) area under the curve (AUC) analysis, and the AUCs were compared using the DeLong test (MedCalc Version 11.6.1.0, Miriakerke, Belgium). ELISA measurements of protein concentrations were normalised by logarithmic transformation for analysis by analysis of variance & 2012 Cancer Research UK
Sensitivity
Invasion assay Cell invasion was studied using a modification of the Cytoselect 24-well Cell Invasion Assay (Cell Biolabs, San Diego, CA, USA). Briefly, 0.5–1.0 106 cells ml 1 were seeded onto the insert chamber of the plate in medium containing 1% FBS, while medium containing 10% FBS was added to the lower chamber of the invasion plate. The plate was incubated at room temperature for 1 h. Purified human plasma-derived ApoC-II (Sigma–Aldrich) or recombinant human SAA-1 (PeproTech, Rocky Hill, NJ, USA) were added to the inner chamber. The plate was incubated for 2448 h at 37 1C in 5% CO2. The non-invading cells were removed and invading cells stained using the cell-stain solution and counted with a light microscope under 10 magnification, with at least three individual fields counted per insert.
Molecular Diagnostics
Abbreviations: ALP ¼ serum alkaline phosphatase; ALT ¼ alanine amino transferase; AST ¼ aspartate amino transferase; CA 19-9 ¼ Carbohydrate Antigen 19-9. Geometric mean values with the inner quartile range; Bilirubin (serum bilirubin), ALP, AST, ALT, CA 19-9. Resection clearance R0, R1, R2 indicate clear margins 41 mm, cancer within 1 mm of the margin and macroscopic tumour at to the margins. Otherwise values are number of cases. Stage I and II indicate the tumour is confined to the pancreas with or without early nodal spread. Stage III indicates the tumour has reached the margin of resection or that there is more extensive nodal spread. Grade I, II and III indicates well, moderate and poor differentiation. ** indicates geometric mean was different from both training and verification sets values by analysis of variance with LSD post hoc analysis, Po0.005.
60
40
20
0 0
20
40 60 100-Specificity
80
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Figure 1 ROC curves for training samples. Dashed line indicates the three-member panel of m/z 3700, 8222 and 11 522 with an AUC of 0.79 (s.e., 0.13). Solid line indicates the panel, including CA 19-9 with AUC of 0.97 (s.e., 0.03). These lines were not significantly different (P ¼ 0.24, DeLong test).
(ANOVA) and survival analysis and the geometric means presented. Post hoc analysis was by the Fishers least-significance difference (LSD) method. To determine the influence of ApoC-II and SAA-1 on survival in Kaplan–Meier analysis of the validationset samples, the cutoff values, which demonstrated the most significant difference between groups were selected. Co-correlation of protein indices with clinical and pathological indices was assessed by Spearman’s test. Univariate and multivariate effects on British Journal of Cancer (2012) 107(11), 1883 – 1891
ApoC-II predicts outcome of resection for PCa A Xue et al
1886 survival were tested using univariate Cox proportional hazard regression analysis and the five most significant independent variables were analysed by backward multivariate regression.
RESULTS Characteristics of patients in the three sets The three patient sets were similar with respect to age, sex, serum and liver enzyme levels with the exception of serum bilirubin,
A i
4000
6000
which was lower in the validation set, probably because of the wider use of stenting in these patients (Table 1). In the 40 trainingset patients, the surgical procedures undertaken were the following: pyloric preservation resection in 28, total pancreatectomy in eight and standard pancreaticoduodenectomy in four. Twenty-nine received adjuvant chemotherapy, six received chemoradiotherapy and five had no adjuvant therapy. Eight patients died from cancer progression within 12 months of surgery. Median survival was 15 months (IQR 11–22 months). Of the verification-set patients, twenty-one underwent a pancreaticoduodenectomy. Six died from cancer progression
B i
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C
Molecular Diagnostics
HPLC SDS-PAGE Serum
6000 m/z
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D
HPLC SDS-PAGE Serum MW (kDa)
ApoC-II
98
10 7.5 5 2.5 0
62
ii 10
i 188
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ii 10 5 0
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17 14 SAA-1 6
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7.5 5 2.5 0
49
ApoC-II 8.2 kDa
6000
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11.5 kDa
iii 10
7.5 5 2.5 0 iv 10 7.5 5 2.5 0
5 0
iv 4 2 0 7500
10 000 m/z
v 4 2 0 10 000
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Figure 2 Purification and identification of the proteins corresponding to m/z 8222 and 11 522 peaks. (Ai) Initial SELDI profile from serum; (Aii) semipurified peak after HPLC fractionation showing the m/z 8222 peak (arrowed) selected for sequencing. (Bi) Initial SELDI for serum; (Bii) semi-purified sample for the m/z 11 522 peak (arrowed). (C) Western-blot lanes are labelled: serum (crude serum), HPLC (the HPLC fraction containing the peaks of interest), SDS–PAGE (the protein extracted from 1D-SDS–PAGE gel bands, which were subjected to protein sequencing and molecular weight (MW) (the protein standards, which are presented in kDa) for ApoC-II in the panel on the left and SAA-1 in the panel on the right. (D) Confirmation of m/z 8222 peak identity using SELDI immuno-adsorption on RS100 chips containing ApoC-II antibody. (Di) Affinity-purified IgG negative control; (Dii) unprocessed serum without antibody; (Diii) unprocessed serum with antibody; (Div) antibody with semi-purified ApoC-II from patient serum. (E) Confirmation of m/z 11 522 peak identity using SAA-1 antibody. (Ei) Affinity-purified IgG (negative control); (Eii) unprocessed serum without antibody; (Eiii) recombinant human SAA-1 (positive control); (Eiv) unprocessed serum with antibody; and (Ev) semi-purified SAA-1 from patient serum after HPLC purification with antibody. British Journal of Cancer (2012) 107(11), 1883 – 1891
& 2012 Cancer Research UK
ApoC-II predicts outcome of resection for PCa A Xue et al
1887
Analysis of the training set of serum samples by SELDI-TOF identified 59 protein peaks with intensities at least 10-fold greater than baseline. Logistic regression and sixfold crossover validation analysis demonstrated that 18 peaks (m/z ¼ 3208, 3432, 3476, 3700, 3879, 4398, 4461, 5051, 5367, 5785, 7333, 8222, 9422, 11522, 12862, 13889, 16963, 17367) along with CA 19-9 were possibly predictive of survival of o1 year. Seven of these peaks and CA19-9 were chosen on two or more occasions in the sixfold cross-validation analysis. Three of these peaks, m/z 3700, 8222, 11 522, were independent of each other by Spearman’s correlation, and AUC for the combination of these peaks to predict 1-year survival was 0.79 (CI, 0.64–0.90). The mean value was greater in those surviving o1 year, but this was only significant for the m/z 8222 peak (P ¼ 0.034). When CA 19-9 was included in the panel (m/z 3700, 8222, 11 522 þ CA 19-9), survival of o1 year was predicted by ROC analysis with AUC ¼ 0.957 (CI, 0.84–0.996; Figure 1). Two patients who were predicted to live 412 months died at 336 and 364 days after surgery. One patient predicted to live o12 months lived for 972 days. The sensitivity of this panel was 0.86 and specificity was 0.82 at the strongest criterion value. When this equation was tested on the SELDI results from the verification set, the six patients predicted to have a poor prognosis died within the first 12 months after surgery, whereas 10 of 15 patients were correctly predicted to live 412 months. The other five died at 21, 26, 45, 50 and 51 weeks after surgery. Thus, although the sensitivity was only 0.55, the specificity was 1.0 and the post-test probability of outcome, given a positive test, was also 1.0.
Identification of the m/z 8222 protein as ApoC-II and the m/z 11 522 protein as SAA-1 Figure 2 illustrates the steps involved in identification of the two proteins m/z 8222 and 11 522 after the ion-exchange fractionation step. The data obtained by LC-ESI/MS/MS showed five major tryptic fragments from the protein peak at m/z 8222, two of them overlapping. MASCOT analysis revealed that the biomarker at 8222 m/z corresponded to ApoC-II with sequence coverage of 82.6%. Two major tryptic fragments were obtained from the protein peak at m/z 11 522, which was identified as SAA-1 with sequence coverage of 47.2%. The intensity of the protein peak at m/z 3700 was too low for identification.
SELDI immuno-adsorption and western-blotting validation Western-blotting analysis was performed for ApoC-II (m/z 8222 peak) comparing crude serum samples, HPLC-purified serum and the excised SDS–PAGE band. A similar comparison was performed for SAA-1 (m/z 11 522 peak). The results showed that the antiApoC-II and anti-SAA-1 antibodies recognised the respective target proteins in purification fractions and SDS–PAGE gel-bands excised for MS/MS analysis. The peaks migrated in a similar manner to the originally targeted proteins (Figure 2C). Other less intense peaks were also seen in the 26 and 90 kDa region, but these were considered to be owing to the polyclonal nature of the antibodies used, although they were purified to be 490% specific for the protein of interest. & 2012 Cancer Research UK
ELISA study on validation-set samples ELISA serum measurements (Table 2) demonstrated that ApoC-II (analysed as geometric means) were lower in resectable cases than in advanced cases (APDCa) (Po0.001) with about half of the values being o10 mg ml 1, the lowest value for HV cases (Figure 3). Serum SAA-1 levels were higher in PDCa serum than APDCa (P ¼ 0.028) or HV samples (P ¼ 0.003) but, CRP was not different among the groups. Kaplan–Meier survival analysis demonstrated that ApoC-II at the cut-off values of 10 mg ml 1 significantly influenced survival (Figure 4, A, P ¼ 0.009), whereas SAA-1 and CRP were not predictive of survival (data not shown). CA 19-9 at a cut-off of 200 IU l 1 (Figure 4B, P ¼ 0.009) and the pathological finding of large-vessel invasion (Figure 4C, P ¼ 0.025) Table 2 Measurement of SAA, ApoC-II, CRP and CA19.9 by ELISA in validation set SAA (ng ml 1) N Mean
ApoC-II (lg ml 1)
CRP (ng ml 1) CA19.9 U l 1
s.d. Mean s.d. Mean s.d. Mean
PDCA 57 104.0 3.9 6.2 2.5 APCA 13 32.9 3.8 15.3 3.7 HV 12 9.9 2.0 13.7 1.6 P by ANOVA 0.000 0.001 P for PDC vs 0.013 0.007 APCA P for PDC vs HV 0.000 0.027
3.7 3.4 2.4 n.s.
4.1 4.3 4.9
105 267 — n.s.
s.d. 4.1 2.1 —
Abbreviations: ANOVA ¼ analysis of variance; APCA ¼ advanced pancreatic carcinoma; HV ¼ healthy volunteers; PDCA ¼ pancreatic ductal adenocarcinoma. Values are geometric means±s.d.
80
60
40
20
0 PDCa
APDCa
HV
Figure 3 Plot of serum concentrations of ApoC-II for the different groups in the Validation study. The horizontal line indicates the cut-off value used for prognosis (10 mg ml 1). British Journal of Cancer (2012) 107(11), 1883 – 1891
Molecular Diagnostics
SELDI-TOF MS derivation of a prognostic panel
Further immunological confirmation that the protein peaks were ApoC-II and SAA-1 was undertaken with SELDI immunoadsorption using commercial ApoC-II and SAA-1 antibodies covalently bound to RS100 chips (Figure 2D and E). Here the m/ z 8222 and 11 522 proteins bound to ApoC-II and SAA-1 antibodies, respectively, with minimal non-specific binding to IgG control. Further, ApoC-II antibody binds to the m/z 8222 peak in unprocessed serum (Figure 2D(iii)) and HPLC-purified serum (Figure 2D(iv)). The SAA-1 antibody bound to the m/z 11 522 peak with a recombinant human SAA-1 sample (Figure 2E(iii)) (positive control), with unprocessed serum (Figure 2E(iv)) and with semipurified SAA-1 (Figure 2E(v)).
Serum apoc-ll (g ml–1)
within 12 months of surgery, whereas the median survival was 17.3 months (IQR 11–23 months). Of the validation-set patients, fifty-seven underwent a pancreaticoduodenectomy with 22 requiring vascular resection, indicating that they had more advanced tumours. Twelve died within 12 months of surgery because of recurrence, with a median of 14.3 months (IQR 10.3–17.6 months).
ApoC-II predicts outcome of resection for PCa A Xue et al
1888
A
1.0
B
0.8 ApoC-ll 10 g ml–1
0.0
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1
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Survival years
C
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0.8
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Molecular Diagnostics
Figure 4 Kaplan–Meier survival curves for factors influencing survival after resection for PDCa in validation-2 samples. (A) The survival was significantly reduced if ApoC-II was 410 mg ml 1 (solid line, n ¼ 28) compared with o10 mg ml 1 (dotted line, n ¼ 29), log rank P ¼ 0.009. (B) The survival curves for CA 19-9 at the cutoff of 200 U l 1 where dotted line represents patients with CA 19-94200 U l 1 (n ¼ 24) and solid line represents patients with values were o200 U l 1 (n ¼ 33), P ¼ 0.009. (C) The influence of pathologically determined large-vessel invasion (dotted line, n ¼ 16) vs rest of the cases (solid line, n ¼ 41) P ¼ 0.025. (D) Combination of the three measures with the solid line representing 15 subjects with three low-risk measures, dotted line indicates survival of 8 subjects with 3 abnormal values, the dashed line indicates survival of 25 subjects with 1 abnormal measure and the dashed-dotted line indicated survival of 9 subjects with 2 abnormal values. Note that three patients with large-vessel involvement had low values of ApoC-II and CA19-9. The curves were different, w2 ¼ 20.4, u ¼ 3, Po0.001.
also demonstrated influence on survival. The combination of these three measures indicated that survival was excellent for the 15 subjects (26%) with all three values in the low-risk range but patients were unlikely to survive 12 months when the three measures were in high-risk range (8 subjects, 14%). The remaining patients with one or two high-risk measures had a median survival of 2 years (Figure 4D).
proportional hazards model using backward regression: log10ApoC-II, log10SAA-1, log10CA19-9, lymph node involvement and large-vessel involvement. The final equation included log10ApoC-II, log10CA19-9 and lymph node involvement for predicting survival (u ¼ 3, w2 ¼ 12.1 and P ¼ 0.007).
Cox proportional hazard assessment of prognostic factors
In-vitro studies of the influence of ApoC-II and SAA-1 on the growth of CFPAC-1 and PANC-I pancreatic cancer cell lines demonstrated a dose-dependent response to 0.05, and 2.5 mg ml 1 of purified ApoC-II and to 25 mg ml 1 of SAA-1 (Figure 5A, ANOVA, Po0.01) but not to 50 mg ml 1 of purified SAA-1 at 24 h. Furthermore, when CFPAC-1 and PANC-1 cells were pre-treated with recombinant ApoC-II or SAA-1 compared with control PBS, before seeding onto Matrigel-coated transwells there was significant increase in cell invasion (Po0.001) in both cell lines but there was no additive effect of combining SAA-1 and ApoC-II (Figure 5B and C).
Multiple potential prognostic factors were assessed by univariate Cox proportional hazard analysis (Supplementary Table S1). This allowed comparison of the proteins of interest, SAA-1 and ApoC-II, with CRP, CA 19-9, serum bilirubin and the ratio of total neutrophil to lymphocytes in the preoperative blood film (Neu/ LCC), along with pathological indices. Spearman’s correlation indicated that ApoC-II was independent of other measures, whereas SAA-1 had a weak but non-significant correlation with the Neu/LCC ratio (P ¼ 0.07), and CA19-9 only correlated with the need for vascular reconstruction. Large-vessel invasion correlated with T-stage (P ¼ 0.03), retroperitoneal nerve invasion (P ¼ 0.02), perineural invasion (P ¼ 0.006) and small-vessel invasion (P ¼ 0.008). Lymph node involvement correlated with T-stage and retroperitoneal extension. Therefore to avoid interference by co-correlation, the following indices were entered into the Cox British Journal of Cancer (2012) 107(11), 1883 – 1891
Impact of SAA-1 and ApoC-II on PDCa cell growth and invasion
DISCUSSION This paper demonstrates that preoperative levels of ApoC-II are prognostic of long-term survival in patients undergoing resection & 2012 Cancer Research UK
ApoC-II predicts outcome of resection for PCa A Xue et al
1889 Cell growth % controls
A
250
CFPANC-1
*
200
*
PANC-1
* *
150 100 50 0 Control
0.05
2.5
10
25
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50
5&50
SAA-1
µg/ml
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B PANC-1
CFPANC-1
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Colonies (number/HPF)
140 120
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**
CFPANC-1 PANC-1
** **
100 80 60 40
*
*
20
*
0 Control
ApoC-ll
SAA-1
Both
Treatment
Figure 5 (A) The influence of ApoC-II and SAA-1 on cell growth (mean values±s.e.m.), as a percentage of controls after 24 h for CFPAC-I (shaded bars), and PANC-I (solid bars). Two-way analysis of variance showed no difference between cell lines. ApoC-II increased cell growth above that of controls (Po0.001 for both cell lines) and greater than the combined treatment with ApoC-II and SAA-1. SAA-1 increased cell growth at the lower dose of 25 mg ml 1 Po0.001, but not at the higher dose 50 mg ml 1, which was less stimulatory than each dose of ApoC-II (Po0.001). ‘*’ indicates different from controls, Po0.001. 5 and 50 indicates 5 mg ml 1 ApoC-II and 50 mg ml 1 SAA-1. (B) Micrograph of 10 magnification transwell invasion assay for PANC-1 and CFPAC-1 cells treated with control solutions, ApoC-1, SAA-1 and both treatments. Bar ¼ 200 mm. (C) Cell counts per high power field at 10 results for 3–4 fields (mean values±s.e.m.). Analysis of variance indicated a significant increase from controls for CFPAC-1 cells (**Po0.001) and for PANC-1 cells (*Po0.02). When treatments were combined, the apparent increase did not reach significance.
for PDCa. The discovery of this protein was undertaken using the high through-put technique of SELDI-TOF MS and logistic regression analysis with sixfold cross-validation. These findings were verified by SELDI in a separate sample set from a different hospital and, were validated by ELISA in a prospective patient group where the patients with a good outcome had depressed levels of ApoC-II. Survival curve analysis by Cox regression of the validation-set samples confirmed that ApoC-II, CA 19-9 and lymph node involvement independently predict survival. Major-vessel involvement, which may be more easily assessed preoperatively with CT scanning (Manak et al, 2009) than lymph node involvement, was shown by Kaplan–Meier analysis to allow for a clear separation of patient outcomes when combined with ApoC-II and CA19-9. The influence of SAA-1, also identified on SELDI & 2012 Cancer Research UK
analysis, was not found to be significant in the validation set. Of interest, SAA-1 and CRP have previously been shown to be inflammatory markers with prognostic utility (Raynes and Cooper, 1983). ApoC-II is mainly synthesised by the liver (Kardassis et al, 2003), and is an important component of LDL, where it helps to solubilise lipid and deliver it to peripheral tissues by its ability to activate lipoprotein lipase (LPL). After the lipid is released, LDL shrinks and ApoC-II splits off either to pass back into the circulation or be utilised by the cancer. Interestingly ApoC-II has been shown to be a putative substrate for the metalloproteinases MMP-14 and MMP-7 (Kim et al, 2006). ApoC-II is efficiently cleaved by MMP-7, which may account for the depressed levels of ApoC-II in our cases with better outcomes (Giannopoulos et al, British Journal of Cancer (2012) 107(11), 1883 – 1891
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2008). Further, pancreatic cancer utilises lipid in preference to carbohydrate (Wang et al, 2009) for energy, which may add to the turnover of ApoC-II. Our finding that ApoC-II increases the growth and spread of pancreatic cancer cells in vitro may indicate a further role of ApoC-II where high levels predicted poorer survival. Thus, although the prognostic importance of ApoC-II has not been previously reported, there are plausible cellular mechanisms involving ApoC-II in the progress of pancreatic cancer. It is interesting that elevated levels of ApoC-II have been considered to be of diagnostic importance (Chen et al, 2007) and associated with pancreatic cancer-associated cachexia (Felix et al, 2011). SAA-1, the second protein selected by SELDI was previously shown to be prognostic of outcome in a number of malignancies related to an induced systemic acute-phase reaction (Weinstein et al, 1984), but, when measured by ELISA in our validation set, it failed as a prognostic marker. This is an unexpected result given that a number of these patients required vascular resection. The cachexia of pancreatic cancer is associated with an increase in inflammatory cytokines and would be expected to increase SAA-1 (Argiles et al, 2009), but few patients with resectable pancreatic cancer have established cachexia (Aslani et al, 2010). Of interest it is a lipoprotein, which is associated with HDL (van der Hilst, 2011) and has a complex, highly conserved, role in acute inflammatory conditions (Manley et al, 2006), which accounts for its elevation in our preoperative patients who had recently had stents for obstructive jaundice (Wagholikar et al, 2003). Recently SAA-1 was included in a diagnostic panel for pancreatic cancer (Bunger et al, 2011). Similarly, the ratio of neutrophils to lymphocytes has intriguingly been associated with poor prognosis (Bhatti et al, 2010), particularly in patients undergoing chemotherapy (Chua et al, 2011), implying an effect on the body’s inflammatory response, although, again we were not able to confirm this. Carbohydrate Antigen 19-9 level of 4200 U ml 1 and lymph node involvement were independently prognostic of poor survival after pancreatic resection (Berger et al, 2004), as confirmed by this study. Although the prognostic value of CA 19-9 has been debated both for (Mehta et al, 2010) and against (Kondo et al, 2010), it does appear to indicate benefit for selection of patients for adjuvant therapy (Hallemeier et al, 2011). Multiple influences on survival include pathological criteria and nutritional and performance status (Schnelldorfer et al, 2008), but CA 19-9 may have further independent predictive value (Tsavaris et al, 2009). However, CA 19-9 results may be confusing because it can be elevated in the presence of biliary inflammation (Dogan et al, 2011) and is not produced in Lewis antigen-negative subjects (Vestergaard et al, 1999). Surgical technique to achieve an R0 resection has an important role in achieving long-term survival (Waraya et al, 2009) where
en-bloc resection allows for clearance of involved vessels (Schnelldorfer et al, 2008). This approach has been used in the current study (Samra et al, 2006). However, margin status continues to be a debated topic and there is a wide belief that the aggressive nature of PDCa over-rides the importance of margin status (Butturini et al, 2008). Major-vessel involvement seen on multi-slice computer tomography is predictive of survival (Manak et al, 2009) and may be used in the preoperative assessment of prognosis along with levels of ApoC-II and CA 19-9. Our results led us to analyse whether ApoC-II and SAA-1 were involved in cancer cell proliferation and invasion. ApoC-II and to a lesser extent SAA-1 stimulated cell proliferation and invasion in two pancreatic cancer cell lines. The apolipoproteins appear to have important roles in the biology of pancreatic cancer. ApoA-II is also depressed in pancreatic cancer (Xue et al, 2010). Although ApoC-II has a different function from ApoA-II, their involvement is consistent with pancreatic cancer’s reliance on lipid metabolism. Further, RELN (Reelin) pathway signalling via the very low-density lipoprotein (VLDL) receptor, to which ApoC-II is known to bind, influences cell motility in pancreatic cancer (Sato et al, 2006). We speculate that ApoC-II levels may be involved with tumour progression. In conclusion, this study shows that serum levels of ApoC-II, and CA 19-9 along with major vessel invasion, which can be determined preoperatively with a CT scan, allow for improved prediction of prognosis of a pancreatic resection. These prognostic indicators selected a small group of short-term survivors, who might have been better served by a palliative procedure, while the remaining patients benefitted from the surgery. Furthermore, these measures provide information about factors leading to a poor prognosis by highlighting the stimulation of cancer tissue growth by apolipoproteins. These factors are so strong that even after the inclusion of the influence of standard histopathological measures and CA 19-9, the blood level of ApoC-II remains a strong independent predictor of survival.
ACKNOWLEDGEMENTS This work was supported by grants from the Cancer Surgery Research Foundation, a Cancer Council NSW Innovator Grant and FIMP (Fondazione Italiana Malattie del Pancreas). The research was facilitated by an initiative of the Australian Government as part of the National Collaborative Research Infrastructure Strategy. We thank Dr Sarah Smith for her editorial assistance. Supplementary Information accompanies the paper on British Journal of Cancer website (http://www.nature.com/bjc)
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