Silver as a Pharmaceutical?

Silver as a Pharmaceutical?

The following paper is a collaboration between Researcher Leonard Paul and Odell Miner, president of LaSal Laboratories, the inventor of the chelated silver solution.

Over the last two decades entrepreneurs and scientists alike have focused on elemental silver as a natural antimicrobial, antibiotic and antisepsis. Research and developmental testing results on silver seem remarkable. Increasingly, many believe silver may have significant potential as a pharmaceutical.

This singular emphasis on silver [Ag] has produced a number of U.S. Patents and Patent Applications. Patent claims on both colloidal and chelated silvers document a variety of viable uses of silver compounds on potentially pandemic microorganisms. Recent tests suggest inter-tumor cancer cells are interdicted by silver compounds.

Claims for both chelated and nano-sized particles of silver are common in todays laboratory test and marketplace. Colloidal silver is ubiquitous in the health food industry and nano particles of silver are now commonly found on bandages and wound dressing products. Silver is actively marketed world-wide as an anti-pathogen technology delivered by foams, mists, spraying solutions and first aid devices.

Silver delivery methods and systems have generated remarkable laboratory results with varying microorganism reduction in ranges from three to six [in some instances, seven] logs. Microorganisms killed include Methicillin-resistant Staphylococcus Aureus (MRSA), Pseudonymous aeroginosa, Escherichia coli, Salmonella choleraesus, and Hepatitis. Viricidal activity is known to be obtained against bacteriophage 029, a small DNA virus; bacteriophage 082, a large DNA virus; and Herpes Virus. Recently in vitro and in vivo laboratory testing seems to have produced promising results in challenging breast and basal skin cancers.




Heightened results are obtained by replacing nano crystal-colloidal [suspended, or particle] forms of silver with chelated silver [silver in molecular solution]."
Silver gels are also reported to keep wounds free from infection over many months. Scientifically verifiable information suggests some forms of silver perform equal to or better than many topical dressings that are commonly used by some clinics and hospitals.

Anti-pathogenic forms of silver, either chelated [molecular silver 'in-solution'] or colloidal ['in-suspension] particles of elemental silver are utilized for preserving corpses, condyloma, and as a lubricant for childbirth. One historical patent related to silver asserts "a difficult infection to combat is caused by herpes virus in the vagina of some women. This infection is persistent and quite resistant to ordinary medication. There is some hint in research work that herpes virus is associated with cervical cancer.

Chelated vs. Colloidal Silver

Fundamental chemistry associated with chelation and colloidal forms of silver is commonly misunderstood by those lacking germane academic, disciplined, or professional training. Colloidal silver is, by definition, finely-divided particles of elemental silver suspended in [pure] water.


Chelated silver is, simply stated, silver in solution, not suspension. The precise chemical definition is: Chelated is a chemical compound having a ring structure that usually contains a metal ion held by coordinate bonds; or or pertaining to a heterocyclic ring containing a metal ion attached by coordinate bonds to at least two nonmetalic ions in the same molecule.


The goal of those who manufacture or promulgate colloidal silver is to reduce the particles of silver as small as possible. The theory is that in so doing greater surface area is achieved; and greater surface area exponentially increases microbial activity. The typical size of colloidal particles is 0.01 to 0.001 micron [a micron = a millionth of a meter. This sizing is commonly accomplished by some type of electrolysis. Total parts per million [ppm] typically reflects the total weight [likely not the concentration] of particles. Commonly commercial products contain between ten and a hundred ppm of silver.

Because they are typically delivered in purified water, colloids are usually ingested [not used topically] for efficacy. Directions on labels suggest taking one or two teaspoons several times daily. In recent years colloidal silver is added in minute amounts to lotions, detergent bases, or sprays for topical applications.

In contrast and because the silver is in solution, chelated silver achieves much higher parts per million – usually around ten to fifty times the ppm of a colloid. Studies document that higher ppm of chelated silver [in solution] substantially increases efficacy of the silver. Chelated silver products are commonly used for topical applications. Although not harmful or toxic in moderate amounts, chelated silver is not intended to be ingested.

Conclusions from research published in June 2004 at Oxford University on therapeutic properties of silver chelates were remarkable.

In order, the Abstract, Methods, Objectives, and Conclusions were:

Abstract: To investigate whether silver chelates or silver ions are more effective as therapeutic agents, and to examine their mode of action so that safer and stable compounds that have a broad spectrum of their therapeutic activities can be developed.

Methods: Efficacy was investigated against pseudonymous aeruginosa (ATCC 15442) by determining MIC via a broth macrodilution procedure using NCCLS methods for antibiotic susceptibility testing.

Results: It was found that the responsible agent for silver therapeutic properties is the silver chelates rather then the silver ions, contradicting previous findings, and the efficacy profiles mimic that of free ions present in solution.
Conclusions: Silver therapeutic activities seem to be more effective as complexes-an inter cellular package-rather then free silver ions, demonstrating that the effect of silver is linked to cells' DNA unwinding and not respiratory or membrane functionality as was traditionally recognized.


Southern Research Institute [SRI] reports and conclusions from prior work by HAP International [Bloomfield, Connecticut] also supports the conclusion that a "strong bonded chelate" would destroy cancer cells. This was the culmination of theory on how chelated silver would destroy cancer cells.

SRI Research & Findings

Does In vitro and In vivo injections of Colloidal Silver and injections of Chelated Silver Kill Cancer Cells? [Two studies – The first was with only colloidal silver]

In vitro and in vivo tests were recently conducted by Southern Research Institute.
Initially [in 2009]. Two different forms of silver were utilized to challenge breast cancer - colloid [nano particles] and "virtual-chelate-gel" silver. The in vitro challenges proved that the silver alone was highly effective down to 6ppm in killing cancer cells with the following results:

Both results proved to be non-toxic when tested in vivo with six mice (results available). A thorough in vivo test challenging breast cancer tumors was conducted. The final report concluded that colloidal silver was only very slightly effective in killing breast cancer cells using both iv and it (direct injection) injections. A simultaneously conducted in vitro challenge on Basel Skin Cancer cells indicated that the chelated silver proved to be just as effective in killing these cells as it did with the breast cancer cells.

The second study in the fall of 2010 with chelated silver).

After considerable research to find and develop a second chelate, we found a Patented Chelated Silver [with silver ions in solution] as compared with NaCl in water! This Patented Chelated Silver [developed at LaSal Laboratories, LLC.] was to become the primary silver chelate. After extensive deliberation it was decided to increase the ppm in gel form of the colloidal silver from 500ppm to 1000ppm. This formulation was to be injected it and orally.
LaSal Labs agreed to increase their patented chelated silver formulation to 420ppm and, subsequently to 840ppm. In consultation with all parties, Southern Research determined to directly inject the 840ppm silver IT. The following protocols and results were reported:

Treatment with nano particles at a dosage of 1000ppm was minimally effective in inhibiting the growth of the MDA-MB-231 human mamory tumor producing a growth delay value of 2.7 days.
Treatment with the chelated silver solution administered at a dosage of 840 ppm was effective in inhibiting the growth OT the MDA-MB-231 human mamory tumor producing a growth delay value of >4.3 days.

Final results proved that the 840ppm silver solution was definitely and startling effective in killing breast cancer tumor cells in vivo. Although the silver gel was not as effective as the silver solution, it proved to be effective when compared to the placebo tumors.


A Senior Project Leader at Southern Research Institute summarized the import of the cancer research:
It has long been known that proliferating cells readily uptake positively charged drugs. Hence, a rapidly dividing cancer cell would be expected to uptake positively charged silver ions more readily than normal cells. Once inside the cell, silver ions would be expected to interact with the cellular DNA causing cell deaths. Silver has been known to be non-toxic for many years. Our studies would suggest that we could deliver silver ions to cancer cells at concentrations high enough to kill the cancer, without causing toxicity to surrounding normal cells. This should be especially true if direct intra-tumoral delivery is used.


Findings suggest that chelated silver destroys tumor/cancers cells. A future challenge will be to formulate a non-toxic ionic silver complex that will kill from a distance IV and orally, beside by direct IT injection in [or through] the bloodstream. That the solution will cure leukemia and lymphomas as well seems reasonably theorized.


UNEDITED LASAL LABS UNEDITED

Questions and Answers that include failed premises that resulted in only using silver complexes for toxicity and in vivo challenges.

(Q) What was the original premise or premises that led to the in vitro, toxicity and in vivo challenges of breast and skin cancer cells/tumors at Southern Research Institute in Birmingham, AL?

Quotes from the first Provisional Patent Application: " This invention teaches that the clinical use of noble metals, combined with the addition or a plurality of other basic metal as active ingredients, for example a Nono-Silver/Gold/Zinc amalgam, would likely destroy any type of cancer. In this example, both Zinc and Silver are not toxic when administered in the proper format into the human/animal body and Gold, in micro amounts, will be tolerated in the form of a cocktail that will invade the cancerous area. The amalgam would enter the blood stream orally via the digestive system or directly via intravenous procedure."

In testing, Copper was added to the list of metals to be used to form complexes/amalgams. All the metals were chelated and mixed in percentages that I felt were apropos for the experiments. However when mixed in solution, any silver/gold chelates could not be used as that combination was not compatible and precipitated. Silver, copper and zinc individually and combination of the three metals were formulated and delivered to SRI for the breast and skin challenges in vitro. Results and conclusions drawn for all these tests can be found in SRI's Dr. Michael Roberts reports within this Web Site. I was advised, by SRI, at that point that toxicity testing of the silver chelate must be performed before live or in vivo challenges of breast cancer tumors should be undertaken. If successful at any parts per million (ppm)of the silver, in vivo (intravenous) and it (intratumoral-directly into the cancer) would begin. The silver chelates eventually were proven to be non-toxic up 1000ppm.

The first breast cancer challenges began with one chelated silver complex along with a control group both iv (250ppm) and it (500ppm) inoculations. SRI's next group of breast cancer challenges that was performed exactly like the first, included a second Patented Chelate that was totally in solution. This second silver chelate proved to be far more successful in killing tumor cells then the first chelate. (See SRI's final report and addendum letter for results and conclusions.)

My own conclusion for the excellent results achieved by the second chelate SRI used in mice, is that it is the only true ionic silver chelate in solution that does not break down its bond eventually in vivo. All chelates will kill gram positive/gram negative bacteria, some viruses, fungi and cancers in vitro and topically but will shortly unbind when introduced into living creatures. A third chelate that can be found on the Internet, claims that its silver's success story is based on the fact that the chelate breaks down so that free ions are active within the blood stream.

Article: http//jac.oxfordjournals.org/content/54/2/546.full






of Dr. Gustavo Doncel at the East Virginia Medical School these tests proved toxic due to the surfactants I used with the foamer technique; many positive challenges that killed gram positive and gram negative bacteria including mrsa tested and certified by the FDA, EPA etc. approved Northeast Lab, Inc., Berlin, CT; three challenges at BYU including using residue dry silver on filter paper that proved positive on both gram positive and gram negative bacteria ( these tests were conducted to prove to Dexter Paper that this process could be used for surgical and anti-pathogen face masks), the process is covered by a Patent; tests were conducted with other anti-pathogens including American Colloid bentonite; once again testing 32ppm colloidal silver gel ageist HIV while also testing the toxicity effect of the silver on the flora in the vagina, these proved to be ineffective as a gel challenging the HIV although not effecting the proactive flora (these tests were conducted by Imquest Lab in Fredericks, MD) and finally Southern Research Institute was contracted to challenge breast and skin cancer with a third and a fourth silver, two different formulated chelated silvers.