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ABSTRACTS / Journal of Molecular and Cellular Cardiology 44 (2008) 711–825
effect in blood pressure lowering. Amlodipine seems to reduce sympathetic tone, while both drugs suppress sBRS. Keywords: Orthostatic hypertension; Telemetry; Antihypertensive drugs
⁎ Corresponding author. Department of Experimental Cardiology, O.O. Bogomoletz Institute of Physiology, 4, Bogomoletz Street, 01024, Ukraine. Tel.: +380 2562013; fax: +380 2562000. E-mail address:
[email protected]
doi:10.1016/j.yjmcc.2008.02.225
Abstract No. 225 Stem cell-derived cardiac patches: A tissue engineering approach to cardiac healing G. Forte ⁎, Carotenuto F., Pagliari F., Pagliari S., P. Cossa, R. Fiaccavento, A. Ahluwalia, G. Vozzi, B. Vinci, A. Serafino, M. Minieri, P. Di Nardo. Rome, Italy ⁎ Corresponding author. Laboratory of Cellular and Molecular Cardiology of the University of Rome “Tor Vergata”, Italy. Tel.: +39 06 72594214; fax: +39 06 72594263. E-mail address:
[email protected] The role of adult stem cells in cardiac homeostasis and repair has been addressed by several studies and the possibility that resident or circulating progenitors could be transplanted into the damaged myocardium envisaged as a new perspective for the treatment of cardiac diseases. So far, few protocols based on the injection of cells into the injured site demonstrated the complexity of a cell-based approach to cardiac repair, suggesting the adoption of tissue engineering concepts to setup more suitable procedures. In the present study, a functional comparison between mesenchymal (MSC) vs. cardiac progenitor cells (CPC) is given by culturing them on biocompatible and bioerodible synthetic scaffolds. In such conditions, both cell types showed multipotential capacity and, when tested for their ability to grow on polymeric scaffolds of PLLA, PLGA and PCL produced by Pressure Assisted Mycrosyringe (PAM), expressed functional adhesion proteins, while preserving their multipotential capacity on 2D polymers. Interestingly, on 3D scaffolds with controlled tridimensional geometry, CPC were prompted to express the cardiac phenotype and formed a quasi-tissue on PLLA polymeric matrix, while MSC preserved their undifferentiated features. Such results demonstrate the feasibility of in vitro stem cell-derived cardiac patches and suggest that it is possible to obtain 3D bio-constructs with different degrees of induction to be used in cardiac tissue healing. Further investigations will be necessary to assess the activity of MSC- and CPC-based patches in vivo.
The aim of the study was to investigate possible differences in the sensitivity of interfibrillar (IFM) and subsarcolemmal (SSM) of the mitochondria in the rat myocardium to Ca2+ induced swelling and their modulation by ω-3 polyunsaturated fatty acid (PUFA). Two fractions of the mitochondria were isolated as described earlier (Palmer, 1977). Mitochondrial swelling was estimated spectrophotometrically by a decrease in the optical density at 520 nm. Two groups of rats were used, the control and experimental one in which rats were fed by ω-3 PUFA (0.1 ml/100 g of epadol containing 45% ω-3 PUFA). In the control group, in a Ca 2+ free solution, the decline in the optical density at the 15th min was not significantly different between SSM and IFM fractions (to 81.43 ± 1.67% and 80.81 ± 2.89%, respectively). However, in the ω-3 PUFA-treated group, the decline in the optical density in the Ca2+ -free solution for the IFM fraction was significantly less pronounced (to 91.88 ± 2.1%) than that for the SSM fraction (to 81.87 ± 1.13%). Furthermore, in the control group, Ca2+ addition to SSM evoked a greater decrease in the optical density for the SSM (to 63.56 ± 5.5% at the 15th min); while in the ω-3 PUFA-treated group this drop in the optical density in the Ca2+ -containing solution for this mitochondrial fraction was significantly (Р b 0.05) less pronounced (to 71.46 ± 0.67%). Ca2+ addition induced a decrease in the optical density to 65.07 ± 6.87% and 83.95 ± 1.62% (Р b 0.05) for the IFM in the control and experimental groups, respectively. These data demonstrate that treatment with ω-3 PUFA provides protection of the mitochondria of both fractions; however, the degree of protection is significantly higher in IFM. Thus, it is concluded that ω-3 PUFA protect the mitochondria against Ca2+ -induced swelling, and the protective effect of ω-3 PUFA is noticeably more pronounced for IFM than for SSM. Keywords: Mitochondria; ω-3 PUFA; Injury doi:10.1016/j.yjmcc.2008.02.227
Abstract No. 227
Keywords: Cardiac stem cells; Tissue engineering; Cardiac disease doi:10.1016/j.yjmcc.2008.02.226
Abstract No. 226 Interfibrillar mitochondria in ω-3 PUFA-fed rats exhibit a higher resistance to calcium than subsarcolemmal ones Olga Panasiuk ⁎, Oleksiy Moybenko. Bogomoletz Institute of Physiology NAS of Ukraine, Ukraine
Massive complement activation in desmin deficient cardiomyopathy M. Mavroidis ⁎, S. Psarras, G. Doumanides, Y. Capetanaki. Biomedical Research Foundation Academy of Athens, Athens, Greece ⁎ Corresponding author. Cell Biology Division, Academy of Athens, Biomedical Research Foundation, Soranou Efesiou 4, Athens 115-27, Greece. Tel.: +30 210 6597057; fax: +30 210 6597545. E-mail address:
[email protected]
