nd 2 Iran
National Zeolite Conference (2INZC2015)
27-28 May 2015, Iran University of Science and Technology Tehran, Iran
Synthesis of nanozeolite A from natural zeolite as a potential carrier for drug delivery Sh.Rahmania, N. Asemia ,S.N.Azizib* aDepartment of chemistry, University of Mazandaran, Babolsar, Iran bDepartment of chemistry, University of Mazandaran, Babolsar, Iran *
[email protected] Abstract Synthesized nanozeolite A was prepared from natural Iranian clinoptilolite (Clin) as Si source and sodium aluminate as Al source using hydrothermal method. The synthesized nanozeolite A was characterized by instrumental analysis methods such as X-ray diffraction (XRD), infrared (IR) and scanning electron microscopy (SEM). In this study adsorption of diclofenac sodium (DS) as a model drug onto nanozeolite A was investigated and Ultra Violet-Visible (UV-Vis) spectrophotometry was used for determination of concentrations of DS. The results demonstrate an interesting potential use of nanozeolite A as drug carrier.
Introduction Porous nanomaterials, such as nanozeolites, are emerging materials for biomedical applications. Zeolites are microporous materials with a threedimensional framework consisting of silicon, aluminum and oxygen. Due to the well-defined pore structure and pore topology, zeolites are widely used for drug delivery [1], adsorption of pharmaceuticals [2] and imaging [3]. Nanoscale zeolites have large surface areas due to appreciable internal and external surface areas [4, 5]. The external surface area of nanoscale zeolites is up to an order of magnitude larger than the external surface area for micron-sized zeolites and provides an additional surface for reaction or functionalization [6]. Therefore, in this study, nanozeolite A synthsized from natural clinoptilolite was used as a drug carrier.
concentration of DS(ppm)
Materials and method Natural clinoptilolite as Si source was purchased from afrazand corporation. Nanozeolite A was synthesized in three steps: 1. Clinoptilolite was dissolved in alkaline solution, 2. The Al solution was prepared from sodium aluminate, 3. The zeolite was synthesized in the hydrothermal conditions and the mixture solution was heated at 90 °C for 2 hours. The product was filtered, washed, dried and characterized by IR, XRD, SEM techniques. Then 2 g of synthesized nanozeolite were soaked, for one day, at room temperature, under continuous stirring, in a DS solution (200 mg/l) in methanol. DS concentrations in the methanol solutions were determined by spectrophotometer (UV–Vis) at 220 nm. The adsorption characteristics of DS on nanozeolite A were examined at different time and also the analysis was carried at two pH levels. Results are shown in Fig. 1. 250 200 pH=7
150 pH=5
100 50 0 0
500
1000
1500
2000 Time(min)
Fig 1. Effect of time and pH on the adsorption of DS
Results and Discussion FT-IR spectrum of the synthesized nanozeolite A is illustrated in Fig. 2. The vibration of T-O bending in 475.42 cm-1, double ring in 576.68 cm-1, and TO4 asymmetric stretch in 1035.7 cm-1 resulted from FT-IR analysis as certain the formation of zeolitic structure. The XRD pattern of the synthesized sample is shown in Fig. 3. This pattern indicated that the synthesized sample has the structure of nanozeolite A. The SEM image of the particles is shown in Fig. 4, which shows uniform particle morphology and small particle size in range of 44-92 nm and it has the cubic structures of SiO4 and AlO4- tetrahedral. From the results in Fig. 2, it is obvious that amount of DS was removed from the solution via adsorption on surface of nanozeolite A.
Fig 2 . FTIR spectrum of synthesized nanozeolite A
Fig 3. XRD pattern of synthesized nanozeolite A
Fig 4. SEM image of synthesized nanozeolite A
Acknowledgment The author would gratefully thank Dr. Farzane Zaerie, pharmacy student at Tehran University of Medical Sciences and Mr Reza Hosseinpour Kouhestany, M.Sc student in analytical chemistry at university of mazandaran for his kind assistance. Conclusions Our study display that clinoptilolite is a suitable Si source for preparing of nanozeolite A and this method is very fast and easy. Adsorption of diclofenac sodium on surface of nanozeolite A was succesfull. The study on the zeolite discussed here demonstrate that this structure can be used effectively for carrier drug applications. This carrier can reduce toxicity and enhance therapeutic efficacy of drug, also can control release of drug in body. References [1] M.G. Rimoli, M.R. Rabaioli, D. Melisi, A. Curcio, S. Mondello, R. Mirabelli, E.Abignente, J. Biomed. Mater. Res, 2008, 156–164. [2] A. Martucci, L. Pasti, N. Marchetti, A. Cavazzini, F. Dondi, A. مراجع Alberti, Microporous Mesoporous Mater, 2012, 174–183. [3] K.J. Balkus, J. Shi, J. Phys. Chem, 1996, 16429–16434. [4] W. Song, V. H. Grassian, S. C. Larsen, Chem. Commun,2005, 2951– 2953. [5] W. Song, R.E. Justice, C.A. Jones, V.H. Grassian, S.C. Larsen, Langmuir, 2004, 4696–4702. [6] W. Song, J.F. Woodworth, V.H. Grassian, S.C. Larsen, Langmuir, 2005, 7009–7014.
