Urinary cell-free DNA as a potential tumor marker for bladder cancer

The International Journal of Biological Markers, Vol. 22 no. 4, pp. 287-294 © 2007 Wichtig Editore

Urinary cell-free DNA as a potential tumor marker for bladder cancer H.W. Chang1,2, K.H. Tsui3, L.C. Shen4, H.W. Huang4,5, S.N. Wang6, P.L. Chang3 1Faculty

of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 2Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 3Chang Gung Bioinformatics Center, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 4Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung 5National Sun Yat-Sen University-Kaohsiung Medical University Joint Center, Kaohsiung 6Division of Hepato-biliary-pancreatico Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung - Taiwan

ABSTRACT: The objective was to assess the possibility of measuring urine creatinine (UCr)-adjusted urinary cell-free (ucf) DNA concentration as a noninvasive screening tool for bladder cancer. Using PicoGreen-based detection, the ucfDNA/UCr concentration was quantified in urine supernatant specimens from 46 bladder cancer patients and 98 controls and compared to 400-bp real-time PCR-based detection, which detected the amplification of 400-bp β-actin (named 400bp ucf-DNA/UCr). The mean concentrations for both PicoGreen and 400-bp ucf-DNA (ng/mL)/UCr (mg/dL) were significantly higher in bladder cancer patients than in controls: 15.28 vs 6.68 (p