A molecular phylogenetic reappraisal of the Didymosphaeriaceae (= Montagnulaceae)

Fungal Diversity (2014) 68:69–104 DOI 10.1007/s13225-014-0305-6

A molecular phylogenetic reappraisal of the Didymosphaeriaceae (= Montagnulaceae) Hiran A. Ariyawansa & Kazuaki Tanaka & Kasun M. Thambugala & Rungtiwa Phookamsak & Qing Tian & Erio Camporesi & Sinang Hongsanan & Jutamart Monkai & Dhanushka N. Wanasinghe & Ausanan Mapook & Ekachai Chukeatirote & Ji-Chuan Kang & Jian-Chu Xu & Eric H. C. McKenzie & E. B. Gareth Jones & Kevin D. Hyde

Received: 18 July 2014 / Accepted: 25 August 2014 / Published online: 26 September 2014 # School of Science 2014

Abstract The ascomycetous families, Didymosphaeriaceae and Montagnulaceae, have been treated in Pleosporales, Dothideomycetes, and both include saprobes, endophytes and pathogens associated with a wide variety of substrates worldwide. Didymosphaeriaceae was characterized by 1septate ascospores and trabeculate pseudoparaphyses, mainly anastomosing above the asci, while species in Montagnulaceae had 1 to multi-septate ascospores and generally cellular pseudoparaphyses. In recent treatments, Bimuria, Didymocrea, Kalmusia, Karstenula, Montagnula, Paraphaeosphaeria, Paraconiothyrium and Letendraea were placed in Montagnulaceae, while only Didymosphaeria has been placed in Didymosphaeriaceae. New morphological and

molecular data from recent collections have become available and thus the understanding of the families can be improved. Based on analyses of concatenated internal transcribed spacer (ITS) with LSU, SSU and β-tubulin gene sequences, the taxonomy of the genera classified in these families are reassessed. Our phylogenetic analyses conclude that, the recently introduced Didymosphaeria rubi-ulmifolii with Pa ra c o ni ot hy r iu m b r as il ie ns e s p e c i e s co m p l e x , Alloconiothyrium, Bimuria, Deniquelata, Didymocrea, Kalmusia, Karstenula, Letendraea, Montagnula, Neokalmusia, Paraconiothyrium, Paraphaeosphaeria, Phaeodothis and Tremateia, forms a robust clade named here D i dy m o s ph a e r i ac e ae . We t h e r e f or e s yn o n ym i z e

Electronic supplementary material The online version of this article (doi:10.1007/s13225-014-0305-6) contains supplementary material, which is available to authorized users. H. A. Ariyawansa : J.