Antimicrobial activities in Sarcotragus spinosulus and Crambe crambe (Porifera, Demospongiae) associated bacteria Presenter: Perino E.1*, Horta A.2, Manconi R.3, Pronzato R.1,Pedrosa R.2. 1
Dipartimento di Scienze della Terra, dell’Ambiente e della Vita (DISTAV), Università di Genova, Corso Europa 26, I-16132 Genova, Italy, *
[email protected]; 2Grupo de Investigação em Recursos Marinhos (GIRM), ESTM, Instituto Politécnico de Leiria (IPL), Peniche, Portugal, 3Dip. di Scienze della Natura e del Territorio (Dipnet), Univ. di Sassari, Via Muroni, 25 - 07100 Sassari, Italy Background Marine sponges continue to attract considerable interest both as excellent natural product sources and as hosts of often highly complex symbiont communities. Current data suggest that at least some of the bioactive compounds from sponges are of bacterial origin. Objective In this study we performed a screening of the potentialities of the Staphylococcus aureus growth inhibition of Crambe crambe and Sarcotragus spinosulus associated bacteria. Material and methods We collected by SCUBA C. crambe and S. spinosulus in the NW Mediterranean Sea. After collection the epiphytic bacteria were isolated and grown. The bacteria biomasses obtained were extracted with DCM:MeOH. The antimicrobial potential of the extracts was evaluated by their ability to inhibit the Staphylococcus aureus growth. Results Discussion We tested 55 sponge associated bacteria and 7 of them presented 100% of S. aureus growth inhibition (1000 µg/ml). The IC50 of four different extracts-induced S. aureus growth inhibition was lower than 100µg/ml. Furthermore, one extract obtained from one of the Crambe crambe associated bacteria revealed an IC50 potency of 10µg/ml. These antimicrobial activities results are very promising, although further analysis will be needed to assess the molecules involved on this antibacterial effect.
