Biological responses of human mesenchymal stem cells to titanium wear debris particles

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Biological responses of human mesenchymal stem cells to titanium wear debris particles Article in Journal of Orthopaedic Research · June 2012 DOI: 10.1002/jor.22002 · Source: PubMed

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Rocky S Tuan

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University of Pittsburgh

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NIH Public Access Author Manuscript J Orthop Res. Author manuscript; available in PMC 2013 June 1.

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Published in final edited form as: J Orthop Res. 2012 June ; 30(6): 853–863. doi:10.1002/jor.22002.

Biological Responses of Human Mesenchymal Stem Cells to Titanium Wear Debris Particles Hana Haleem-Smith1, Evan Argintar1,2, Curtis Bush1,2, Daniel Hampton1,2, William F. Postma1,2, Faye H. Chen1, Todd Rimington1,2, Joshua Lamb1,2, and Rocky S. Tuan1,2,3,* 1Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Service, Bethesda, MD 20892 2Department

of Orthopaedic Surgery, Georgetown University School of Medicine, Washington,

DC 20007 3Center

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for Cellular and Molecular Engineering, and Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219

Abstract

NIH-PA Author Manuscript

Wear debris-induced osteolysis is a major cause of orthopaedic implant aseptic loosening, and various cell types, including macrophages, monocytes, osteoblasts, and osteoclasts, are involved. We recently showed that mesenchymal stem/osteoprogenitor cells (MSCs) are another target, and that endocytosis of titanium (Ti) particles causes reduced MSC proliferation and osteogenic differentiation. Here we investigated the mechanistic aspects of the endocytosis-mediated responses of MSCs to Ti particulates. Dose-dependent effects were observed on cell viability, with doses >300 Ti particles/cell resulting in drastic cell death. To maintain cell viability and analyze particle-induced effects, doses