Cranio-maxillofacial trauma: A 13 year review of almost 12.500 cases

Oral Presentations / 02. Trauma I and reduced disease-free (P =0.0004) and overall (P =0.0179) survival. On the other hand, p16 deletion was detected in 39%(22/57). There was a significant association between p16 deletion and histopathologic grading (P =0.012), disease recurrence (P =0.038). However, the diseasefree and overall survival rates for patients with p16 deletion was lower than that of patients without p16 deletion, although this just failed to reach statistical significance (P = 0.0516 and P = 0.1878). p 16 deletion in the presence of cyclin D1 numerical aberration conferred a significantly worse disease-free (P = 0.0002) and overall (P =0.0153) survival. Analysis of the cyclin D1 numerical aberration and p16 deletion using FISH on FNA biopsies is a simple and sensitive method for predicting tumors aggressiveness, recurrence, and clinical outcome in patients with oral SCCs. References [1] Miyamoto R, Uzawa N, Nagaoka S, et al.: Potential marker of oral squamous cell carcinoma aggressiveness detected by fluorescence in situ hybridization in fine-needle aspiration biopsies. Cancer 2000; 95: 2152-2159. [2] Miyamoto R, Uzawa N, Nagaoka S, et al.: Prognostic of cyclin D1 amplification and overexpression in oral squamous cell carcinomas. Oral Oncology 2003; 39: 610-618.

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EXPRESSION OF CD 44 IN ORAL SQUAMOUS CELL CARCINOMAS

regulatory pathway of cAMP metabolism modulated by cGMP. However, no data are available as to the expression of PDE2 in melanoma cells. In this study, we examined the expression of PDE2 in human melanoma cell lines. Cell lines: Human melanoma cell lines, PMP, HMV-II, G-361 and C32 were used. PMP cells were established from a patient with palatal melanoma and maintained in our laboratory. HMV-II and G-361 cells were purchased from the RIKEN CELL BANK, and C32 cells from DAINIPPON PHARMACEUTICAL CO., LTD. PDE2 activity: PMP cells were seeded at 1.5x 106 cells/100 mm dish, HMV-II and C32 cells at 3 x 106 cells/100 mm dish, and G-361 cells at 1 x 106 cells/25 cm 2 flask. After 3 days, the cells were harvested and homogenized in 1 ml of ice-cold homogenization buffer. PDE activity was measured with/without Erythro-9-(2-Hydroxy-3nonyl)adenine hydrochloride (EHNA), a specific inhibitor of PDE2, and cGMP, a stimulator of PDE2. RT-PCR: Total RNA was extracted from the cells by the RNeasy ® Mini Kit, and first-strand cDNA was generated from total RNA byTaqman ® Gold RT-PCR kit. Using primer pairs specific for human PDE2A, PCR amplification was carried out by HotStarTaqTM DNA Polymerase Reagents. In PMP cells PDE activity was stimulated by cGMP and inhibited by EHNA. In HMV-II, G-361 and C32 cells PDE activities were slightly inhibited by EHNA. PDE2A mRNA was detected by RT-PCR in RNA from PMP and HMV-II cells, but not from G-361 and C32 cells. These data indicated that PDE2 was expressed in human melanoma PMP and HMV-II cells, but not in G-361 and C32 cells. To establish whether PDE2 inhibitors may be useful as an antitumor drug in the treatment of melanoma, we will study the effect of PDE2 inhibitor on the growth of these cells.

A.W. Eckert, M.H.W. Lautner, P. Maurer, U. Bilkenroth, S. Hauptmann, J. Schubert. Department of Oral and Maxillofacial Plastic Surgery,

Martin-Luther-University, Halle-Wittenberg D-06097 Hafle/Saale ErnstGrube-Str. 40, Germany CD 44 is a transmembrane glycoprotein, which belongs to the family of molecules that adhere to cell surface. This protein is important in the interaction between cells and matrices. It was discovered recently, that CD 44 may play a role in the metastasis and prognosis of tumors. Since the overall 5-year survival in oral squamous cell carcinoma [OSCC] remains poor it was the aim of the present pilot study to investigate the relationship between CD 44 expression and clinical outcome in OSCC. Series of 41 OSCC cases with known clinical outcome were divided into 2 groups: group 1 presented the better clinical outcome (survival more than 5 years, no recurrence), group 2 summarised all patients with less survival or with recurrence. Immunohistochemical staining for CD 44 was carried out using a standard immunoperoxidase technique at paraffin-embedded specimen. All stainings were viewed by three investigators and a semiquantitative immunoreactice score [REMMELE] was applied. In group 1 12 cases were graded to have a negative or a weak staining reaction, 11 cases were dark positive stained for CD 44. In contrast, only 7 cases were found to be moderate or intensive in the group with poor clinical outcome. There was a significant relationship between CD 44 expression and the stage of the tumor disease [ChiQuadrat = 3.92, p