Esophageal atresia and associated cardiovascular anomalies

AM AGA ABSTRACTS

GASTROENTEROLOGY Vol. 118, No.4

523

525

BRUSH BORDER SUGAR TRANSPORTER EXPRESSION IS ALTERED FOLLOWING MASSIVE SMALL BOWEL RESECTION. Brian M. Chung, Laurie E. Wallace, Bob Winkfein, James A. Hardin, Ted V. 0' Loughlin, Donald G. Gall, Univ of Calgary, Calgary, AB, Canada; New Childrens Hosp, Sydney, Australia. Following resection, the distal remnant small bowel displays decreased in vitro net glucose transport and brush border membrane (BBM) glucose uptake kinetics. Oral epidermal growth factor (EGF) returns in vitro net glucose transport to control levels despite having no effect on BBM glucose uptake kinetics. Hypothesis: Small bowel resection alters the expression of the BBM sugar transporters SGLTI and GLUT5. Methods: NZW rabbits (lkg) underwent a 70% small bowel resection. One group of resected animals received oral EGF (40 J.Lglkg, days 3-8 post surgery), and were compared to untreated resected, and unmanipulated controls. 10 days post-surgery, in separate experiments, BBM were prepared from distal remnant tissue for Western immunoblots, whole mucosa for Northern blots, or tissue fixed for microscopic localization of SGLTl and GLUT5 along the villus. Results: Resection decreased BBM SGLTl content compared to controls and was not altered by oral EGF (SGLTl CON .56::'::.03, RES .42::'::.02, R-EGF .41::'::.03, n=5, p