Heterologous Aspergillus niger β-galactosidase secretion by Saccharomyces cerevisiae

Abstracts / Journal of Biotechnology 131S (2007) S196–S210 Harbison, C., Gordon, D., Ihn Lee, T., Rinaldi, N., Macisaac, K., Danford, T., Hannett, N., Tagne, J.-B., Reynolds, D., Yoo, J., Jennings, E., Zeitlinger, J., Pokholok, D., Kellis, M., Rolfe, P., Takusagawa, K., Lander, E., Gifford, D., Fraenkel, E., Young, R., 2004. Transcriptional regulatory code of a eukaryotic genome. Nature 431, 99–104.

doi:10.1016/j.jbiotec.2007.07.353 7. Excretive overproduction of microbial polyglucosamine PGB-1 of Enterobacter sp. BL-2 in acetate-mediated culture condition through metabolic engineering of hexosamine pathway Mi-Kyung Son ∗ , Soo-Jung Hong, Yong-Hyun Lee Kyungpook National University, 702-701 Daegu, South Korea A new bacterial strain Enterobacter sp. BL-2 excretively produced a unique cationic polyglucosamine biopolymer PGB-1 comprised of more than 95% d-glucosamine in acetate-mediated culture condition. The biopolymer PGB-1 could be synthesized from the UDP-N-acetylglucosamine monomer derived from the hexosamine pathway. Three glmS, glmM, and glmU genes in the hexosamine pathway were cloned from Enterobacter sp. BL-2, and their molecular structures were elucidated. The cloned glmS, glmM, and glmU genes were re-enforced into the parent strain Enterobacter sp. BL-2 through a conjugative transformation for the excretive overproduction of the biopolymer PGB-1. The production of the biopolymer PGB-1 increased 1.5-fold in the transformant Enterobacter sp. BL-2S over-expressing the firststep glmS gene encoding glucosamine-6-phosphate synthase. The transformant Enterobacter sp. BL-2S was also cultivated pH-stat fed batch wisely, while feeding the acetate solution intermittently to maintain a constant pH level of 8.0 for 72 h, and 1.15 g/L of the extracellular polyglucosamine biopolymer PGB-1 were obtained. doi:10.1016/j.jbiotec.2007.07.354 8. Towards a yeast cell factory platform for polyketide production Anna Eliasson Lantz ∗ , Songsak Michael Lynge Nielsen, Jens Nielsen

Wattanachaisaereekul,

Center for Microbial Biotechnology, Technical University of Denmark, Building 223, DK-2800 Kgs. Lyngby, Denmark Polyketides represent one of the largest groups of natural products being produced by bacteria, fungi and plants. Polyketides represents an important group of bioactive compounds with a remarkably wide structural and functional diversity. More than 0.3% of the known polyketides have been commercialized, which is far higher than the typical hit rate of