Compo P"rasiwl.
77(1),2010, I'p. 25-30
Metacercariae of Clinostomum (Trematoda: Digenea) from Three Species of Ambystoma (Caudata: Ambystomatidae) from Arkansas and lllinois, U.S.A. CHRIS T. McALLlSTER,I,6 CHARLES
R.
COI~EY
TRAUTH:;
SHAFFER,:; AND STANLEY
E.
BLIRSEy,2 JOlIN
A.
CRAWFORD,3 ANDREW
R.
KUNNS,4
RapidWrite, 102 Brown Street, .Hot Springs Nation,i) Park, Arkansas 71913, U.S.A. (e-mail:
[email protected]).
Department of Biology. Pennsylvania Stale University, Shenango Campus. Sharon, Pennsylvania 16146, U.S.A.
(e-mail:
[email protected]),
3 Departmenl of Biological Sclmces, Lindenwood Univer. ity, SI. Charles, Missouri 63301, U.S.A.
(e-mail:
[email protected]).
4 Illinois Naturaillistory Survey, 1816 S. Oak Street, Champaign, Il1inois 61X20, U.S.A. (c-mail:
[email protected]). and
5 DepHrtmelll of Biological Science.s, Arkansas Statc UniversilY, Statc University, Arkansas 72467, U.S.A.
(e-mail:
[email protected])
J
2
AIlSTRAn: Thirteen of 5 I (25.5%) Jefferson salamanders, Ambysloma je./fersonial/um, and 4 of 69 (5.8%) sm,lll-mouthed
salamanders, Ambystoma lexmzum. from east-central Illinois were found to be infected with metaeercariae of Clillostomum
mdtgirwillm; L of 72 (1.4%) spotted salamanders, Ambystol1llJ muculatum from north-central Arkansas was found to be
infected with metaeercariae of Clilloslomum atlellll(J/um. We provide a new host record for C. altenualum and 2 new host
records and a new locality record for melacercariae of C "/(Il'gina/um. In addition. a list of the amphibian hosts of
metacercuriae of ClinOSTOnrui1! 'Pp. is provided.
KEY WORDS: Arkansas, lIIinois. U.S.A.. Amby.l'toma je!fersoniollum. AmhysfOma macula/um, Amhysloma tcxallllfll. AmbyStomatidac, Caudata, Clillo.l'Jomum attel1u{fwfJl, Clil/o.l'lomum (-,omplanalum, ClilllJ.I'wmum mUl'kinatunl, Digenea, metacercariae, Trematoda, Jefferson salamander, small-mouthed salamandct', spolted salamander.
Metacercariae of Clinoswmum spp. have been repot1ed from a variety of North American salaman ders, namely, the 3-toed amphiuma, Amphiuma {ridGetylum from Louisiana (Bennett and Humes. 1938); barred tiger salamander, Amhystorna mavor rium from Texas (Miller et aI., 2004; Gray et aL, 2(07); cave salamander, ElI1ycea lucifuga from Tennessee (McAllister ei aI., 2(07); Texas salaman der, Ewycea neolenes from Texas (McAllister, 1990); eastern newt, No{ophlhalmwr viridescens from Massachusetts (McAllister, .1990) and Michigan (Muzzall, 1991 a); and greater siren, Siren lacertina from Florida (Manter, 1938). I-l.erein. we prav ide I new host record for ClinoslOmllm ottenlloturn and 2 new 'hOSt records and a neW locality record for metacercariae of Clinoslomum marginalum. In addi tion, a list of the North American amphibian hosts of metacercariae of ClinMlomum spp. is provided (Table 1).
Pond. Stone County, Arkansas, U.S.A. (36.0000'N, 92.1 X05 c W). Fifty-one (46 males, 5 female~, SVL "" 70 86 mm) Jefferson salamanders. Amhys/oma je'/j'ersonial1um (Green, 1827), and 59 ~malJ-mouthed salamanders, Ambys toma 1l'X(J/Il1m (Matthes, 1855) (35 males, 24 females; SVL = 70-119 mm), were collected bet ween 12 and 19 March 2008 with un baited minnow Iraps from Zone 16 in Clark County. IIIinoi~. U.S.A. (39.1948"N, 87.4724·'W). All salamanders wilh suspected yellow grub (ClinostomllJ11 spp.) infection were returned to the laboratory and euthanized with a dilute Chloretone@ (chlorobutanol) solution. Lesions (Fig. I) were excised to reveal Ihe unknown worms (Fig. 2). which were fixed unflattened in 10% neutral buffered fornlalin, transferred to 70% ethanol, stained with Semichon's ace!()Cannine, and mounled in Canada Balsam. Parasite idenlifications were based on lhese >50 cxci~ed worms. Host voucher specimens are deposilcd in the Arkansas State University Herpetological Museum (ASUMZ). State University. Arkansas. U.S.A., as ASUMZ 30933 (A. mawlalum), 31144 (A~ je.!fer.l'olliallllm). and 31143 (A. texa/lwn). Pamsile vouchcrs were deposited in the U.S. National Parasite Collection (USNPC). Beltsville, Maryland, U.S.A. as USNPC WI 161-101163. Amphibian taxonomy in Table I follows Frost (20011).
MATERIALS AND METHODS
RESUl.TS
Seventy-two (46 males. 26 females, snout-vent length rSVLl = 69-101 mm) spotted salamanders, Ambystonla mlll'ulalum (Shaw, 1802) were collected on 5 and 19 February 2008 with 19-1iicr buekd pilfall traps from Stout
All excised lesions contained clinosromid meta cercariae of various sizes and ages. A total of 18 of 198 (9.1 %) ambystonlatid salamanders, including 13 of 51 (25.5%) A. jeffersonianum and 4 of 69 (5.8%) A. leXGnum from east-central Illinois were found to be
(, Corresponding aUlhor. 2:;
26
COMPARATIVE PARASITOLOGY, 77(1 l. JANUARY 20!O
Table 1.
Clinostomum spp. in North American amphibians.
Parasile/Hosl
Locality
Prevalence (llCreemage)*
Reference
ClillOSlomum sp. (melacercaria) IImphillma Iridliclylul/I
Louisiana
NoropI1l1111lm"s l'iridesc~IIJ LitllObot"J berlandiai
Mi~higan
Lilho/Jall'S catesheianus
MXt Arizona Mi~higan
l,irhoblJll'.\· chiricahllellsis LilhohlJtes clamitalls
not stated Aril1ma Michigan
l.ilhollllll'S pipiens
Wisc{)Jlsin Michigan
Pspudac";,'1 regilill Rana aurora Raila I>oylii RhillelJa marilla Xenopu... loel'i...
Calif(lmi~1
MXt Califomia California AM; California
6/H5 (7) 12/163 (7) not staled
1/25 (4) 1/127 (I) 6/11 (55) 1/25 (4) 8/239 (3) 1/26 (4) 8/43 (19) 2/128 (2) 10/130 (8) occaslonal occasional
1/167 «I) 49/68 (72)
Bennett and Humes (1938) Mtlzzall (1991 a) Leon-Regagnon ct al. (200S) Goldherg el al. (1998) Mtlzzall (199Ib) Lemke cl al. (2008) Goldberg et al. (\998) Muzzall el al. (2001) Yoder ct al. (2001) Gilliland and MllZzall (1999) Goldherg and Bursey (2001) (Joldberg et al. (200]) Ing\e~ ( (936) Ingles (193/i) Linzey el "I. (I Y98) Kuperman el al. (2004)
C/ino.\'/omum atlelUWIWll
Ambysroma macula/11m Amby,\'/oma ma\'Orl;um
Arkansas Texas Texas Texas MXt Florida Kansas CN§ Michigan MXt Indiana Michigan Minnesolil Iowa Oklahoma Texas Texas
12/39 (3)) 1/1 (100) 3/177 (2) 2/2 (100) 4/370 (I) 1/38 (3) 7/11 (64) 179/41,665 « I)
This study Miller el al. (2004) Gray el al. 2007) Miller el "I. (2()04) Gray el al. (2007) E~es (1991) Creel "I 'II. (2000) Jinks and Jobmon (1971) CO]·t (19131 Najarian (1955) Goldberg and BtlT>
