O.150 CK 17 in oral squamous cell carcinoma cells by brush biopsy

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Journal of Cranio-Maxillofacial Surgery 36(2008) Suppl. 1

Tuesday, 9 September 2008, 16.00–17.00

Sala Italia

Cancer biology and epidemiology I O.147 cAMP-phosphodiesterase signal in malignant melanoma cells Y. Watanabe, T. Murata, K. Shimizu, K. Mizoi, J. Nomura, T. Tagawa. Department of Oral and Maxillofacial Surgery, Division of Reparative and Regenerative Medicine, Institute of Medical Science, Mie University Graduate School of Medicine 1, Tsu, Japan Objectives: Cyclic AMP (cAMP) acts as a second messenger in cells and modulates many physiological processes. Intracellular cAMP content is regulated through synthesis by adenylate cyclase and degradation by phosphodiesterases (PDEs). However, no data are available as to the cAMP-PDE signal in malignant melanoma cells. In this study, we examined cAMP-PDE signal in malignant melanoma cell line. Methods: Cell growth: Mouse B16 melanoma cells were plated at 100 cells/well in a 96-well plate and allowed to adhere for 24 h. The cells were cultured in the absence or presence of different concentration of cAMP analog, 8-bromo cAMP, or cGMP analog, 8-bromo cGMP, for 6 days. The cell numbers were calculated using CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI). ˚ cells/25oz. flask. PDE activity: B16 cells were seeded at 1×10A After 3 days, the cells were incubated with 8-bromo cAMP for 15 min, harvested and homogenized in 1 ml of ice-cold homogenization buffer. PDE activity was measured. Results: 8-bromo cAMP inhibited the growth of B16 cells, but not 8-bromo cGMP. And PDE activity in B16 cells was stimulated by 8-bromo cAMP. Conclusions: These data suggested that cAMP-PDE singal was related with the growth of malignant melanoma cells and cAMPPDE singal might be target for an antitumor drug of malignant melanoma. O.148 Chemical surgery for oral cancer S. Kim. Sacred Heart Hospital, Anyang, Republic of Korea Objective: The control of microinvasion is highly important to improve complete cure rate in oral cancer. We wanted to evaluate the therapeutic effects of chemical agent that has sharp threshold in inducing apoptosis of cancer. Methods: Eight patients were included in this clinical trials and six patients might have remained cancer in resection margin. Topical 4-HR application was done for 1 month postoperatively. Results: Seven patients showed tumor free and mean follow-up was 32 months. There was no metastatic tumor after operation. Conclusion: Though this was a preliminary trials, the chemical surgery could be considered as an additive technique to improve cure rate of oral cancer. O.149 Choice of operation volume in glossal cancer T1-3NxMO R. Bakirov, S. Osokin, R. Osokina. Bashkir Clinical Oncology Health Center, UFA, Russia The aim of our research is to evaluate effectiveness of lymph node dissection as an approach which allows giving prognosis of metastasizing in remote postoperative period. We analyzed history cases of 256 patients with glossal cancer T1−3NxMo who received surgical treatment in Head and Neck Tumor Department. We found out that patients who received treatment only within gemiglossectomy had regional metastases in jugular lymph nodes in 28% of cases.

Abstracts, EACMFS XIX Congress The second group of patients is those who received treatment of initial lesion having simultaneous removing of regional lymph drain ducts whose metastasizing process wasn’t proved by the results of diagnostic study. In this group of patients the frequency of metastases to remote lymph nodes was 23%. In 88.4% of operated patients of this group there were no metastases within one year. The patients of the third group in 10.5% of cases had metastases within one year after operation. Simultaneous regional lymph node dissection and operation of the initial lesion T1−3NxMo can be treated as a factor of surgical prognosis of further course of disease. Morphological research can essentially affect further treatment tactics. If micro metastases without lymph kinesis failure are found patients can remain under dynamic observation. In case of lymph kinesis failure, radical operation on jugular lymph drain ducts is necessary.

O.150 CK 17 in oral squamous cell carcinoma cells by brush biopsy T. Toyoshima, F. Koch, P. Kaemmerer, W. Wagner, B. Al-Nawas. Oral and Maxillofacial Surgery, Johannes Gutenberg University, Mainz, Germany Objectives: Brush biopsy has proved as a non-invasive methodology in diagnosis of oral lesions. Cytokeratin (CK) has been known to be a diagnostic and prognostic marker for solid tumors. The aim of this study was to evaluate the relevance of CKs for diagnosis of oral squamous cell carcinoma (OSCC) using brush biopsy. Methods: Fifty-two pairs of OSCC cells and normal oral mucosal cells were obtained by brush biopsy from OSCC patients. mRNA was extracted from cell pellets for real-time quantitative reverse transcriptase polymerase chain reaction (RT qPCR). The overexpression levels of CK 17, CK 19, and CK 20 in OSCC cells were examined by SYBR green real-time RT qPCR. Results: Compared to in normal mucosal cells, the overexpression of CK 17 was detectable in 40 OSCC cells (76.9%), CK 19 in 19 (36.5%), while that of CK 20 was not detectable in any cells. Compared to the mean value of CK 19 expression level, in all 52 patients, the value of CK 17 was significantly higher (P < 0.02). Moreover, the value of CK 17 was also significantly higher in T1 and T2 OSCC patients (P < 0.03), in patients without metastases of neck lymph nodes (P < 0.02), in stage I and II patients (P < 0.03 and P < 0.05, respectively), and in well differentiated OSCC patients (P < 0.05). Conclusion: The brush biopsy serves for the detection of CK mRNA using real-time RT qPCR. CK 17 could be a promising marker for OSCC diagnosis in brush biopsy test.

O.151 DNA-image cytometry improves assessment of resection margins J. Handschel, C. Sproll, R. Depprich, A. Zimmermann, S. Braunstein, N.R. K¨ubler, U. Meyer, A. B¨ocking. Department for Cranio- and Maxillofacial Surgery, D¨usseldorf, Germany Background: Despite the histopathological findings of tumor free margins, patients with oral squamous cell carcinoma (SCC) often suffer from local tumor relapse. The purpose of this study was to determine the prognostic value of DNA-image cytometry in the assessment of resection margins. Methods: DNA-image cytometry was performed in 40 SCCpatients with histologically tumor-free resection-margins. The follow-up period since the tumor resection was at least 3 years. Results: 20 patients showed a locoregional relapse of the SCC. 14 of these patients had aneuploid cells in DNA-image cytometry. Two patients who were relapse free revealed aneuploid cells too. The sensitivity of the adjuvant use of DNA-image cytometry was 70% and the positive predictive value was 87.5%.