Plasma DNA Integrity Index as a Potential Tumor Marker in Breast Cancer Patients

Plasma DNA integrity index as a potential molecular diagnostic marker for breast cancer Azza M. Kamel, Salwa Teama, Amal Fawzy & Mervat El Deftar

Tumor Biology Tumor Markers, Tumor Targeting and Translational Cancer Research ISSN 1010-4283 Tumor Biol. DOI 10.1007/s13277-015-4624-3

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Author's personal copy Tumor Biol. DOI 10.1007/s13277-015-4624-3

ORIGINAL ARTICLE

Plasma DNA integrity index as a potential molecular diagnostic marker for breast cancer Azza M. Kamel 1 & Salwa Teama 2 & Amal Fawzy 1 & Mervat El Deftar 3

Received: 9 October 2015 / Accepted: 10 December 2015 # International Society of Oncology and BioMarkers (ISOBM) 2015

Abstract Plasma DNA integrity index is increased in various malignancies including breast cancer, the most common cancer in women worldwide; early detection is crucial for successful treatment. Current screening methods fail to detect many cases of breast cancer at an early stage. In this study, we evaluated the level of plasma DNA integrity index in 260 females (95 with breast cancer, 95 with benign breast lesions, and 70 healthy controls) to verify its potential value in discriminating malignant from benign breast lesions. The criteria of the American Joint Committee on Cancer were used for staging of breast cancer patients. DNA integrity index was measured by real-time PCR. DNA integrity index was significantly higher in breast cancer than in benign breast patients and healthy subjects (P =