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International Conference on Challenges in Chemistry and Biology of Carbohydrates
CARBO - XXVIII
January 20-22, 2014 Dehradun INDIA
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ACCTI AS SO
ABSTRACTS
Organized by
ASSOCIATION OF CARBOHYDRATE CHEMISTS & TECHNOLOGISTS (INDIA)
P-82
CARBO-XXVIII 2014
PRECISE STRUCTURAL STUDIES OF TINOSPORA SINENSIS POLYSACCHARIDE Shipra Nagar, Y.C. Tripathi, Vineet Kumar Chemistry Division Forest Research Institute, Dehradun-248 006, India Email:
[email protected]
Tinospora genus is widely used in Ayurveda, Siddha, Unani, Indian and Chinese folk and medicinal systems, for a number of therapeutic applications. With a view to study the structureactivity relationship, the structure of polysaccharide isolated from Tinospora cordifolia has been extensively studied as a highly branched macromolecular structure containing arabinogalactan and glucan backbones with pharmacological activity. Tinospora sinensis, a sister species is widely used independently in therapeutics and also as a substitute of T. cordifolia without any discrimination. Intriguingly, there is no report even towards isolation of the polysaccharide from T. sinensis despite wide medicinal spectrum. With this milieu, fine structural analysis of T. sinensis has been undertaken in laboratory. CWSP and HWSP were isolated in yield 9.1% and 2.1% respectively. Complete hydrolysis followed by GLC analysis of alditol acetates indicated L-rhamnose, L-arabinose, D-mannose, Dgalactose and D-glucose in molar ratio 0.178,0.259,0.140,1.045 and 98.377 in CWSP and Lrhamnose, L-arabinose, D-xylose, D-mannose, D-galactose and D-glucose in molar ratio 0.608,0.628,0.978,0.227,0.111 and 97.448 in HWSP. The uronic acid content was studied by assorted approaches. It was estimated by spectrophotometric methods using chromogens carbazole, m-hydroxydiphenyl and 3,5-dimethylphenol and the results were corroborated by GLC analysis. The studies noticeably brought to light, the efficacy of chromogens and also revealed to what extent, they could be relied upon. In T. sinensis, the galacturonic acid content was reported as 1.41% and 1.20% in CWSP and HWSP respectively. Methylation studies, periodate oxidation and other degradative techniques for studying the linkages in polysaccharide chains are in progress.
International Conference on Challenges in Chemistry and Biology of Carbohydrates!
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