Molecular cloning

Eukaryotic expression: developments for structural proteomics

Proteomics / Gene expression / Biological Sciences / Animals / Physical sciences / Glycosylation / CHEMICAL SCIENCES / Molecular cloning / Yeasts / Eukaryotic Cells / Glycosylation / CHEMICAL SCIENCES / Molecular cloning / Yeasts / Eukaryotic Cells

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Crystal Structure of Mycobacterium tuberculosis Catalase-Peroxidase

Thermodynamics / Molecular Biology / Protein Science / Biological Chemistry / Multidisciplinary / Macromolecular X-Ray Crystallography / Biological Sciences / Crystal structure / Tuberculosis / Mycobacterium tuberculosis / Mutation / Circular Dichroism / Escherichia coli / Mice / Female / Animals / Fluconazole / Cell Death / Physical sciences / Drug Design / Spectrophotometry / Heme / Membrane transport proteins / Enzyme / CHEMICAL SCIENCES / Electrons / Molecular cloning / Active site / Protein Secondary Structure Prediction / Lysine / X ray diffraction / Time Factors / Antifungal Agents / Protein Conformation / Growth Inhibition / Amino Acid Sequence / Protein Binding / Hydrolysis / Cytoplasm / Ligands / Enzyme Catalysis / Protein Transport / Biochemistry and cell biology / Solvents / Dimerization / Adenosine Triphosphate / Adenosine Diphosphate / Molecular Sequence Data / Macromolecular X-Ray Crystallography / Biological Sciences / Crystal structure / Tuberculosis / Mycobacterium tuberculosis / Mutation / Circular Dichroism / Escherichia coli / Mice / Female / Animals / Fluconazole / Cell Death / Physical sciences / Drug Design / Spectrophotometry / Heme / Membrane transport proteins / Enzyme / CHEMICAL SCIENCES / Electrons / Molecular cloning / Active site / Protein Secondary Structure Prediction / Lysine / X ray diffraction / Time Factors / Antifungal Agents / Protein Conformation / Growth Inhibition / Amino Acid Sequence / Protein Binding / Hydrolysis / Cytoplasm / Ligands / Enzyme Catalysis / Protein Transport / Biochemistry and cell biology / Solvents / Dimerization / Adenosine Triphosphate / Adenosine Diphosphate / Molecular Sequence Data

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Novel Dehalogenase Mechanism for 2,3-Dichloro-1Propanol Utilization in Pseudomonas putida Strain MC4

Kinetics / Carbon / Magnetic Resonance Spectroscopy / Multidisciplinary / Energy Metabolism / Electoral Studies / Applied Environmental Microbiology / Oxidoreductases / Pseudomonas putida / Molecular cloning / Substrate Specificity / acrolein / Molecular Sequence Data / Electoral Studies / Applied Environmental Microbiology / Oxidoreductases / Pseudomonas putida / Molecular cloning / Substrate Specificity / acrolein / Molecular Sequence Data

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The UDP N-Acetylgalactosamine 4Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence

Biological Sciences / Lipopolysaccharide / Infection and immunity / Virulence / Humans / Sequence alignment / Escherichia coli / Movement / Mice / Animals / Infection / Molecular cloning / Aeromonas Hydrophila / Amino Acid Sequence / Oncorhynchus Mykiss / Serotyping / Carbohydrate Sequence / Molecular Sequence Data / Sequence alignment / Escherichia coli / Movement / Mice / Animals / Infection / Molecular cloning / Aeromonas Hydrophila / Amino Acid Sequence / Oncorhynchus Mykiss / Serotyping / Carbohydrate Sequence / Molecular Sequence Data

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Lys80p ofSaccharomyces cerevisiae, previously proposed as a specific repressor ofLYS genes, is a pleiotropic regulatory factor identical to Mks1p

Microbiology / Industrial Biotechnology / Transcription Factors / Saccharomyces cerevisiae / Yeast / Molecular cloning / Lysine / Molecular cloning / Lysine

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Subspecific kDNA probes for major clones of Trypanosoma cruzi

Biological Sciences / Animals / Nucleic acid hybridization / Polymerase Chain Reaction / Chagas disease / Molecular cloning / Trypanosoma Cruzi / DNA probes / Molecular cloning / Trypanosoma Cruzi / DNA probes

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Subspecific kDNA probes for major clones of Trypanosoma cruzi

Biological Sciences / Animals / Nucleic acid hybridization / Polymerase Chain Reaction / Chagas disease / Molecular cloning / Trypanosoma Cruzi / DNA probes / Molecular cloning / Trypanosoma Cruzi / DNA probes

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Lys80p ofSaccharomyces cerevisiae, previously proposed as a specific repressor ofLYS genes, is a pleiotropic regulatory factor identical to Mks1p

Microbiology / Industrial Biotechnology / Transcription Factors / Saccharomyces cerevisiae / Yeast / Molecular cloning / Lysine / Molecular cloning / Lysine

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Zebrafish spata2 is expressed at early developmental stages

Developmental Biology / Computational Biology / In Situ Hybridization / Biological Sciences / Spermatogenesis / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Molecular Sequence Data / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Molecular Sequence Data

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Zebrafish spata2 is expressed at early developmental stages

Developmental Biology / Computational Biology / In Situ Hybridization / Biological Sciences / Spermatogenesis / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Molecular Sequence Data / Animals / Male / Polymerase Chain Reaction / Proteins / tESTIS / Molecular cloning / Protein Secondary Structure Prediction / Zebrafish / Amino Acid Sequence / Base Sequence / Molecular Sequence Data

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Natural CD4+ T-cell responses against Trypanosoma cruzi KMP-11 protein in chronic chagasic patients

Immunology / Immune response / Humans / Mice / Female / Animals / Male / Chagas disease / Immunology & Cell Biology / Middle Aged / Molecular cloning / Trypanosoma Cruzi / Major histocompatibility complex / Biological markers / Protozoan Proteins / Membrane Protein / Biochemistry and cell biology / Animals / Male / Chagas disease / Immunology & Cell Biology / Middle Aged / Molecular cloning / Trypanosoma Cruzi / Major histocompatibility complex / Biological markers / Protozoan Proteins / Membrane Protein / Biochemistry and cell biology

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Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4

Kinetics / Carbon / Magnetic Resonance Spectroscopy / Multidisciplinary / Energy Metabolism / Electoral Studies / Applied Environmental Microbiology / Oxidoreductases / Pseudomonas putida / Molecular cloning / Substrate Specificity / acrolein / Molecular Sequence Data / Electoral Studies / Applied Environmental Microbiology / Oxidoreductases / Pseudomonas putida / Molecular cloning / Substrate Specificity / acrolein / Molecular Sequence Data

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AhpC (alkyl hydroperoxide reductase) from Anabaena sp. PCC 7120 protects Escherichia coli from multiple abiotic stresses

Abiotic Stress / Gene expression / Copper / Escherichia coli / Genetic transformation / Cloning / Molecular cloning / RT PCR / Physiological Stress Markers / Anabaena / Biochemistry and cell biology / Peroxiredoxins / Cloning / Molecular cloning / RT PCR / Physiological Stress Markers / Anabaena / Biochemistry and cell biology / Peroxiredoxins

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Mamestra configurata serpin-1 homologues: cloning, localization and developmental regulation

Zoology / Gene expression / Western blotting / Animals / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Glycosylation / Molecular cloning / Western blot / Moths / Amino Acid Profile / Insect Biochemistry and Molecular Biology / Nervous System / Molecular weight / Amino Acid Sequence / Base Sequence / Gene Family / Serine Protease / Low molecular weight / Insect Biochemistry / cDNA library / Biochemistry and cell biology / Molecular Sequence Data / Glycosylation / Molecular cloning / Western blot / Moths / Amino Acid Profile / Insect Biochemistry and Molecular Biology / Nervous System / Molecular weight / Amino Acid Sequence / Base Sequence / Gene Family / Serine Protease / Low molecular weight / Insect Biochemistry / cDNA library / Biochemistry and cell biology / Molecular Sequence Data

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Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor

Genetics / Gene expression / Genetic Engineering / Streptomyces / Industrial microbiology / Spectral analysis / Biochemical / Plasmids / Molecular cloning / Liquid Chromatography / Biotransformation / Gel electrophoresis / Base Sequence / Acetone / Lovastatin / Biochemistry and cell biology / Molecular Sequence Data / Spectral analysis / Biochemical / Plasmids / Molecular cloning / Liquid Chromatography / Biotransformation / Gel electrophoresis / Base Sequence / Acetone / Lovastatin / Biochemistry and cell biology / Molecular Sequence Data

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CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1, 6-glucan assembly

Bacteriology / Membrane Proteins / Western blotting / Biological Sciences / Saccharomyces cerevisiae / Mutation / Mycotoxins / Microbial genetic and drug resistance / Endoplasmic Reticulum / P-glycoprotein / Glycoproteins / Phenotype / Glucans / Molecular cloning / Beta-Glucans / Amino Acid Sequence / Base Sequence / Cell Wall / Molecular Sequence Data / Mutation / Mycotoxins / Microbial genetic and drug resistance / Endoplasmic Reticulum / P-glycoprotein / Glycoproteins / Phenotype / Glucans / Molecular cloning / Beta-Glucans / Amino Acid Sequence / Base Sequence / Cell Wall / Molecular Sequence Data

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